Multiplex transport and detection of cytokines using kinesin-driven molecular shuttles

The application of biomolecular active transport systems offers a potential route for downscaling multiple analyte assays for lab-on-a-chip applications. Recently, the capture and transport of a wide range of target analytes including proteins, virus particles, and DNA have been demonstrated using k...

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Bibliographic Details
Published in:Lab on a chip 2009-01, Vol.9 (7), p.1005-1010
Main Authors: Rios, Lynnette, Bachand, George D
Format: Article
Language:English
Subjects:
Antibodies, Immobilized
Biological Transport
Biotinylation
Cytokines - analysis
Interleukin-2 - analysis
Kinesin - chemistry
Microchip Analytical Procedures
Microtubules - chemistry
Molecular Motor Proteins - chemistry
Nanostructures
Nanotechnology - methods
Streptavidin - chemistry
Tumor Necrosis Factor-alpha - analysis
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Summary:The application of biomolecular active transport systems offers a potential route for downscaling multiple analyte assays for lab-on-a-chip applications. Recently, the capture and transport of a wide range of target analytes including proteins, virus particles, and DNA have been demonstrated using kinesin-driven molecular shuttles. The molecular shuttles consisted of microtubule (MT) filaments that were functionalized with either analyte-selective antibodies or complementary DNA, thus facilitating selective target capture and transport. In the present work, we have applied this microfluidic platform for the simultaneous detection of multiple target protein analytes. Multiplexing of molecular shuttles was achieved by immobilizing biotinylated antibodies against interleukin-2 (IL-2) and tumor necrosis factor-alpha (TNF-alpha) on biotinylated MTs using a streptavidin bridge. Nanocrystal quantum dots of different sizes and spectral emissions were functionalized with IL-2 and TNF-alpha antibodies to facilitate multiplexed detection. In this paper we discuss the results of selectivity and motility in single and multiplexed assays.
ISSN:1473-0197
1473-0189
DOI:10.1039/b816444d