Adenosine, an Identified Active Component from the Driselase-Treated Fraction of Rice Bran, Is Effective at Improving Metabolic Syndrome in Stroke-Prone Spontaneously Hypertensive Rats

• Published in: Journal of agricultural and food chemistry 2009-03, Vol.57 (6), p.2558-2564
• Main Authors: Ardiansyah, Shirakawa, Hitoshi, Shimeno, Taku, Koseki, Takuya, Shiono, Yoshihito, Murayama, Tetsuya, Hatakeyama, Eiko, Komai, Michio
• Format: Article
• Language: English
• Subjects: adenosine; Adenosine - administration & dosage; Adenosine - isolation & purification; adiponectin; Animals; Biological and medical sciences; blood chemistry; blood glucose; Blood Glucose - analysis; blood lipids; carbohydrate metabolism; Driselase; enzymatic treatment; enzymes; Food Chemistry/Biochemistry; Food industries; Fundamental and applied biological sciences. Psychology; Fungal Proteins - pharmacology; gene expression; glucose; Glycoside Hydrolases - pharmacology; hypertension; Hypertension - drug therapy; lipid metabolism; Male; messenger RNA; Metabolic Syndrome - drug therapy; nitric oxide; Nitric Oxide - blood; O-glycoside hydrolases; oral administration; Oryza - chemistry; Rats; Rats, Inbred SHR; rice bran; Seeds - chemistry; Seeds - metabolism; triacylglycerols; Triglycerides - blood
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf803347c

In the present study, we isolated and identified an active component from the Driselase-treated fraction and investigated its effect by acute and chronic oral administration on hypertension, lipid, and glucose metabolism in stroke-prone spontaneously hypertensive rats. The active component was identified as adenosine and improves hypertension after single oral administration. Rats who were 10 weeks old were divided into control and adenosine groups and were administered water or water with adenosine (10 mg/L), respectively, for 3 weeks. Hypertension and plasma lipid, nitric oxide, insulin, leptin, adiponectin levels, and glucose metabolism were significantly improved in the adenosine group. The mRNA expression levels of genes involved in lipid and glucose metabolism were altered in the adenosine group. Single oral administration of adenosine (10 mg/kg body weight) improved hypertension and plasma triglyceride, glucose, and nitric oxide levels 2 h after administration. In conclusion, oral acute and chronic administration of adenosine are beneficial and improve the metabolic syndrome-related disease parameters.