Use of the P Gene to Genotype Human Metapneumovirus Identifies 4 Viral Subtypes

This study, conducted during 2001-2003, undertook the screening of patients with acute infectious respiratory-tract disease. A random selection of positive specimens was used for sequencing studies of the human metapneumovirus (hMPV) nucleoprotein gene and the phosphoprotein (P) gene. Australian and...

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Bibliographic Details
Published in:The Journal of infectious diseases 2004-12, Vol.190 (11), p.1913-1918
Main Authors: Mackay, Ian M., Bialasiewicz, Seweryn, Waliuzzaman, Zubair, Chidlow, Glenys R., Fegredo, David C., Laingam, Somprasong, Adamson, Penny, Harnett, Gerald B., Rawlinson, William, Nissen, Michael D., Sloots, Theo P.
Format: Article
Language:English
Subjects:
Acute Disease
Australia - epidemiology
Biological and medical sciences
Child
Child health services
Family Practice
Female
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetic screening
Genotype
Hospitals
Human metapneumovirus
Humans
Infections
Infectious diseases
Male
Medical research
Medical sciences
Metapneumovirus
Metapneumovirus - classification
Metapneumovirus - genetics
Microbiology
Molecular Epidemiology
Molecular Sequence Data
Nucleoproteins - genetics
Paramyxoviridae Infections - epidemiology
Pathology
Phosphoproteins - genetics
Phylogeny
Respiratory diseases
Respiratory Tract Infections - epidemiology
Respiratory Tract Infections - virology
Sequence Alignment
Sequencing
Specimens
Viruses
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Summary:This study, conducted during 2001-2003, undertook the screening of patients with acute infectious respiratory-tract disease. A random selection of positive specimens was used for sequencing studies of the human metapneumovirus (hMPV) nucleoprotein gene and the phosphoprotein (P) gene. Australian and international sequences were compared, and a global classification scheme was developed. The hMPV P gene was an ideal molecular target for the detection and genotyping of hMPV. The region contained conserved sequences for reverse-transcriptase-polymerase chain-reaction primers and adequate variability to permit the accurate genotyping of the virus into 2 main lineages and 4 sublineages. Establishing viral identity is essential for the linking of genotype and disease severity.
ISSN:0022-1899
1537-6613
DOI:10.1086/425013