Involvement of Human Small Fragment Nuclease in the Resistance of Human Cells to UV-C–induced Cell Death

Human small fragment nuclease (Sfn) is one of the cellular proteins that were reported to degrade small, single-stranded DNA and RNA. However, the biological role of Sfn in cellular response to various stressors such as UV-C (mainly 254 nm wavelength ultraviolet ray) remains unclear. We have examine...

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Bibliographic Details
Published in:Photochemistry and photobiology 2004-09, Vol.80 (2), p.281-285
Main Authors: Ito, Seiro, Kita, Kazuko, Zhai, Ling, Wano, Chieko, Suzuki, Toshikazu, Yamaura, Akira, Suzuki, Nobuo
Format: Article
Language:English
Subjects:
14-3-3 Proteins
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Caspase 3
Caspases - metabolism
Cell death
Cell Death - drug effects
Cell Death - radiation effects
Cell Line
Exonucleases - genetics
Exonucleases - metabolism
Exoribonucleases
Humans
Kinetics
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Pyrimidine Dimers - metabolism
Research s
RNA Interference
RNA, Messenger - genetics
RNA, Messenger - metabolism
Ultraviolet Rays
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Summary:Human small fragment nuclease (Sfn) is one of the cellular proteins that were reported to degrade small, single-stranded DNA and RNA. However, the biological role of Sfn in cellular response to various stressors such as UV-C (mainly 254 nm wavelength ultraviolet ray) remains unclear. We have examined whether modulation of human SFN gene expression affects cell survival capacity against UV-C–induced cell death, analyzing colony survival ability in UV-C–sensitive human RSa cells treated with short double-stranded RNA (siRNA) specific for SFN messenger RNA (mRNA). The expression levels of SFN mRNA in the siRNA-treated RSa cells decreased to about 15% compared with those in the control siRNA-treated cells. The siRNA-treated RSa cells showed lower colony survival and higher activity of caspase-3 after UV-C irradiation than the control siRNA-treated RSa cells. Furthermore, the removal capacity of cyclobutane pyrimidine dimers (CPD) in the siRNA-treated RSa cells decreased compared with the control siRNA-treated RSa cells. There was no difference in the colony survival and CPD removal capacity after UV-C irradiation between the control siRNA-treated RSa cells and mock-treated RSa cells. These results suggest that SFN expression is involved in resistance of RSa cells to UV-C–induced cell death through the roles it plays in the DNA repair process.
ISSN:0031-8655
1751-1097
DOI:10.1562/2004-01-21-RA-051.1