Improvement of a latex agglutination test for the evaluation of oxacillin resistance in coagulase-negative staphylococci

The “Slidex MRSA Detection” test (Denka Seiken, Japan) is a latex agglutination assay able to detect PBP2a. We evaluated its ability to differentiate mecA-positive from mecA-negative coagulase-negative staphylococci. We included 100 coagulase-negative staphylococci clinical isolates belonging to 9 s...

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Published in:Diagnostic microbiology and infectious disease 2004-11, Vol.50 (3), p.223-225
Main Authors: Corso, A., Soloaga, R., Faccone, D., Gagetti, P., Corbella, S., Iglesias, M., Galas, M.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Infectious diseases
Latex Fixation Tests - methods
Medical sciences
Microbiology
Oxacillin - pharmacology
Penicillin Resistance - genetics
Penicillin-Binding Proteins - analysis
Reagent Kits, Diagnostic - microbiology
Sensitivity and Specificity
Species Specificity
Staphylococcus - classification
Staphylococcus - drug effects
Staphylococcus - genetics
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cited_by cdi_FETCH-LOGICAL-c503t-f4a1029a83cc5feb38912647c9941fa46f142f7073e90b7ba45e308105b53fae3
cites cdi_FETCH-LOGICAL-c503t-f4a1029a83cc5feb38912647c9941fa46f142f7073e90b7ba45e308105b53fae3
container_end_page 225
container_issue 3
container_start_page 223
container_title Diagnostic microbiology and infectious disease
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creator Corso, A.
Soloaga, R.
Faccone, D.
Gagetti, P.
Corbella, S.
Iglesias, M.
Galas, M.
description The “Slidex MRSA Detection” test (Denka Seiken, Japan) is a latex agglutination assay able to detect PBP2a. We evaluated its ability to differentiate mecA-positive from mecA-negative coagulase-negative staphylococci. We included 100 coagulase-negative staphylococci clinical isolates belonging to 9 species, 54 mecA positive and 46 mecA negative, as characterized by PCR. The specificity achieved using the manufacturer's instructions was 100%, but the sensitivity was only 57%. To increase sensitivity, we introduced modifications into the standard protocol. Using either large inocula or oxacillin induction before test performance, we achieved 100% sensitivity.
doi_str_mv 10.1016/j.diagmicrobio.2004.06.005
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source ScienceDirect Journals
subjects Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Infectious diseases
Latex Fixation Tests - methods
Medical sciences
Microbiology
Oxacillin - pharmacology
Penicillin Resistance - genetics
Penicillin-Binding Proteins - analysis
Reagent Kits, Diagnostic - microbiology
Sensitivity and Specificity
Species Specificity
Staphylococcus - classification
Staphylococcus - drug effects
Staphylococcus - genetics
title Improvement of a latex agglutination test for the evaluation of oxacillin resistance in coagulase-negative staphylococci
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