Molecular cloning and characterization of two novel human RXRα splice variants

Two novel cDNAs encoding RXRα splice variants (RXRα2 and RXRα3) were identified among human full-length cDNA libraries. RXRα2 and RXRα3 cDNAs possess open reading frames, leading to production of proteins lacking the N-terminal 27 and 97 amino acid residues of the RXRα1 product, respectively. RXRα2...

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Bibliographic Details
Published in:The Journal of steroid biochemistry and molecular biology 2004-09, Vol.92 (1), p.19-28
Main Authors: Kojo, Hitoshi, Tajima, Kaoru, Fukagawa, Masao, Isogai, Takao, Nishimura, Shintaro
Format: Article
Language:English
Subjects:
Alternative Splicing - drug effects
Alternative Splicing - genetics
Amino Acid Sequence
Base Sequence
Biological and medical sciences
Cell Line
Cells, Cultured
Cloning, Molecular
Coactivator
DNA, Complementary - genetics
Full-length cDNA library
Fundamental and applied biological sciences. Psychology
Gene Library
Humans
Molecular Sequence Data
Nicotinic Acids - pharmacology
Organ Specificity
Retinoid X Receptor alpha - chemistry
Retinoid X Receptor alpha - genetics
Retinoid X Receptor alpha - metabolism
RXRα
Sequence Alignment
Sequence Homology, Amino Acid
Splice variant
Tetrahydronaphthalenes - pharmacology
Transcription, Genetic - drug effects
Transcription, Genetic - genetics
Vertebrates: endocrinology
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Summary:Two novel cDNAs encoding RXRα splice variants (RXRα2 and RXRα3) were identified among human full-length cDNA libraries. RXRα2 and RXRα3 cDNAs possess open reading frames, leading to production of proteins lacking the N-terminal 27 and 97 amino acid residues of the RXRα1 product, respectively. RXRα2 and RXRα3 genes have respective 5′-terminal exons. RXRα3 is expressed in brain, spleen and prostate whereas the expression of RXRα2 was below the detectable level. Both RXRα2 and RXRα3 showed a level of transcriptional activity and a dose response curve against the agonist LG100268 similar to RXRα1 in reporter assay for the RXRα homodimer or that for the heterodimer with PPARγ2. However, clear differences were observed among the splice variants when dose response curves were compared by the assay in the presence of coactivators such as SRC-1 and PGC-1. These results suggest specific physiological roles of two novel human RXRα splice variants.
ISSN:0960-0760
1879-1220
DOI:10.1016/j.jsbmb.2004.07.001