The oxidation metabolites of endomorphin 1 and its fragments induced by free radicals

Endomorphin 1 (EM1), an endogenous µ‐opioid receptor agonist, acts as a free radical scavenger in vitro and an antioxidant in vivo. The modification of EM1 by ROS and the properties of the OM attracted our attention. In vitro assays were performed via RP‐HPLC, spectrophotometric measurements, EPR an...

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Bibliographic Details
Published in:Journal of peptide science 2009-05, Vol.15 (5), p.337-344
Main Authors: Gong, Pin, Chen, Fu Xin, Zhao, Qianyu, Ma, Guo Fen, Wang, Rui
Format: Article
Language:English
Subjects:
Animals
Brain - metabolism
Cell Membrane - metabolism
Chromatography, High Pressure Liquid
Electron Spin Resonance Spectroscopy
EM1-melanin
endomorphin 1
Free Radicals - chemistry
Free Radicals - metabolism
Male
NAD - chemistry
NAD - metabolism
Oligopeptides - chemistry
Oligopeptides - metabolism
Oxidation-Reduction
oxidative metabolite
Rats
Rats, Wistar
ROS
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Summary:Endomorphin 1 (EM1), an endogenous µ‐opioid receptor agonist, acts as a free radical scavenger in vitro and an antioxidant in vivo. The modification of EM1 by ROS and the properties of the OM attracted our attention. In vitro assays were performed via RP‐HPLC, spectrophotometric measurements, EPR and amino acid analysis, Schmorl's reaction to define the formation of melanin‐like compounds transformed from EM1, collectively named EM1–melanin and by solubility assay, radioligand‐binding assay, NADH oxidation, superoxide anion scavenging assay to study some physical and chemical properties of EM1–melanin. Possible pathways of the formation of EM1–melanin were proposed. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.
ISSN:1075-2617
1099-1387
DOI:10.1002/psc.1116