Discovery of a pimaricin analog JBIR-13, from Streptomyces bicolor NBRC 12746 as predicted by sequence analysis of type I polyketide synthase gene

• Published in: Applied microbiology and biotechnology 2009-05, Vol.83 (1), p.127-133
• Main Authors: Komaki, Hisayuki, Izumikawa, Miho, Ueda, Jun-ya, Nakashima, Takuji, Khan, Shams Tabrez, Takagi, Motoki, Shin-ya, Kazuo
• Format: Article
• Language: English
• Subjects: Antifungal Agents - chemistry; Antifungal Agents - isolation & purification; Antifungal Agents - metabolism; Applied Genetics and Molecular Biotechnology; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bioactive compounds; Biological and medical sciences; Biotechnology; Cluster Analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Enzymes; Fundamental and applied biological sciences. Psychology; Genes; Gram-positive bacteria; JBIR-13; Life Sciences; Metabolites; Microbial Genetics and Genomics; Microbiology; Molecular Sequence Data; Multigene Family; Natamycin - analogs & derivatives; Natamycin - chemistry; Natamycin - isolation & purification; Natamycin - metabolism; NMR; Nuclear magnetic resonance; Phylogeny; Pimaricin; Polyene macrolide; Polyketide Synthases - genetics; Polyketide Synthases - metabolism; Sequence Analysis; Sequence Analysis, DNA; Sequence Homology; Spectrum Analysis; Streptomyces; Streptomyces - genetics; Streptomyces - metabolism; Streptomyces bicolor; Studies; Type I polyketide synthase
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-008-1849-8

Sequence analysis of ketosynthase domain amplicons from Streptomyces bicolor NBRC 12746T revealed the presence of previously unreported type I polyketide synthases (PKS-I) genes. The clustering of these genes with the reference PKS-1 sequences suggested the possibility to produce a polyene compound similar to pimaricin. Thus, the cultured sample from NBRC 12746T was analyzed for the production of polyene compounds. The strain produced an antifungal compound which displayed the UV absorption spectrum of tetraene macrolides. The structure determination based on the spectroscopic analysis of the purified compound resulted in the identification of a novel pimaricin analog JBIR-13 (1). This study therefore strongly suggested that a careful analysis of PKS-I genes can provide valuable information in the search of novel bioactive compounds within a class predicted from phylogenetic analysis.