Constant Light Exposure Causes Dissociation in Gonadotrophin Secretion and Inhibits Partially Neuroendocrine Differentiation of Leydig Cells in Adult Rats

• Published in: Reproduction in domestic animals 2004-12, Vol.39 (6), p.417-423
• Main Authors: Ortega, HH, Lorente, JA, Mira, GA, Baravalle, C, Salvetti, NR
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Differentiation - radiation effects; Cells; Chromogranin A; Chromogranins - analysis; Embryology: invertebrates and vertebrates. Teratology; Endocrine system; Follicle Stimulating Hormone - secretion; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Leydig Cells - chemistry; Leydig Cells - cytology; Leydig Cells - radiation effects; Light; Luteinizing Hormone - secretion; Male; Neurosecretory Systems - chemistry; Neurosecretory Systems - cytology; Phosphopyruvate Hydratase - analysis; Photoperiod; Rats; Rats, Wistar; Rodents; S100 Proteins - analysis; Substance P - analysis; Synaptophysin - analysis
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2004.00541.x

Contents The objective of this work was to study the changes that occur in the Leydig cells of rats exposed to continuous light. The laboratory rat is considered a non‐photoperiodic species because exposure to short photoperiod has little or no effect on the reproductive status. However, exposure of adult female rats to constant light induces polycystic ovaries, indicating that extreme changes in the photoperiod affect the reproductive function seriously. Adult male rats were placed under continuous light conditions for a duration of 15 weeks. After this period, the animals were killed and testicles were dissected and processed by routine histologic protocols. Follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) serum levels were determined by radioimmunoassay (RIA). The visualization of antigens was achieved by the streptavidin‐peroxidase immunohistochemical method. Antibodies against chromogranin A, S‐100 protein, P substance, synaptofisin, neurofilament protein‐200, gliofibrillary acidic protein and neurone‐specific enolase were used. The mean LH serum concentration was significantly lower, while the mean FSH level was significantly higher in treated animals. The expression of S‐100, NSE, CrA, SP and SYN was significantly lower in treated animals. In conclusion, the constant light exposure acting directly at the pituitary level decreases LH secretion. The increased FSH secretion may be due to a partial reduction of the negative androgen feedback in the pituitary gland. Moreover, the constant light exposure affects the expression of some immunomarkers in Leydig cells, possibly because of the changes found in the gonadotrophin level and feedback mechanism.