Synergism and negative interference during co-infection of tomato and Nicotiana benthamiana with two bipartite begomoviruses

Abstract In Brazil, at least eight begomoviruses including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV) infect tomatoes. ToYSV symptoms in tomato and Nicotiana benthamiana appear earlier and are more severe compared to those of ToRMV. We investigated the role of several fa...

Full description

Saved in:
Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2009-05, Vol.387 (2), p.257-266
Main Authors: Alves-Júnior, Miguel, Alfenas-Zerbini, Poliane, Andrade, Eduardo C, Esposito, Débora A, Silva, Fábio N, da Cruz, Ana Cláudia F, Ventrella, Marília C, Otoni, Wagner C, Zerbini, F. Murilo
Format: Article
Language:English
Subjects:
Begomovirus
Begomovirus - pathogenicity
Begomovirus - physiology
Brazil
Geminivirus
Host-Pathogen Interactions
host-pathogen relationships
Infectious Disease
Lycopersicon esculentum
Nicotiana - virology
Nicotiana benthamiana
pathogenicity
Plant Diseases - virology
plant viruses
Plant–virus interaction
protoplasts
Solanum lycopersicum - virology
synergism
tissue distribution
Tomato
Tomato rugose mosaic virus
Tomato yellow spot virus
tomatoes
ToRMV
ToYSV
Viral Interference
Virulence
virus replication
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract In Brazil, at least eight begomoviruses including Tomato rugose mosaic virus (ToRMV) and Tomato yellow spot virus (ToYSV) infect tomatoes. ToYSV symptoms in tomato and Nicotiana benthamiana appear earlier and are more severe compared to those of ToRMV. We investigated the role of several factors in this differential adaptation. To analyze infection kinetics, a single leaf was inoculated and subsequently detached after different periods of time. Viral DNA accumulation was quantified in plants, viral replication was analyzed in protoplasts, and tissue tropism was determined by in situ hybridization. Results indicate that ToYSV establishes a systemic infection and reaches a higher concentration earlier than ToRMV in both hosts. ToRMV negatively interferes with ToYSV during the initial stages of infection, but once systemic infection is established this interference ceases. In N. benthamiana , ToYSV invades the mesophyll, while ToRMV is phloem-restricted. During dual infection in this host, ToYSV releases ToRMV from the phloem.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2009.01.046