Histological analysis in shoot organogenesis from hypocotyl explants of Kandelia candel (Rhizophoraceae)

In vitro culture of hypocotyl explants from Kandelia candel, a common mangrove species, on hormone-free Murashige and Skoog (MS) medium resulted in shoot formation. Since the hypocotyls showed good potential for in vitro shoot multiplication, the process of bud primordium formation was analyzed from...

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Bibliographic Details
Published in:Journal of plant research 2004-12, Vol.117 (6), p.457-464
Main Authors: Ogita, S. (Toyama Prefectural Univ., Kosugi (Japan). Faculty of Engineering), Yeung, E.C, Sasamoto, H
Format: Article
Language:English
Subjects:
Cellular biology
Histology
Hypocotyl
HYPOCOTYLS
Meristem - growth & development
ORGANOGENESIS
Plant biology
Plant growth
Plant Shoots - growth & development
RHIZOPHORACEAE
Rhizophoraceae - anatomy & histology
Rhizophoraceae - growth & development
Seedlings - growth & development
SHOOTS
Tissue Culture Techniques
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Summary:In vitro culture of hypocotyl explants from Kandelia candel, a common mangrove species, on hormone-free Murashige and Skoog (MS) medium resulted in shoot formation. Since the hypocotyls showed good potential for in vitro shoot multiplication, the process of bud primordium formation was analyzed from a histological viewpoint. A wound periderm first appeared at the top, exposed cut surface of the explants. The wound-induced meristem continued to divide giving rise to suberized cells oriented towards the cut surface. After formation of the suberized cell layers. the meristem and its inner derivatives differentiated into multilayered, uniformly packed parenchyma cells. Bud primordia differentiated from the dense cytoplasmic cells of the wound-induced meristem just beneath the suberized layer near the severed vascular bundles. Each explant produced several visible shoot buds. Furthermore, histological sections revealed that additional bud primordia were present within the explant just underneath the suberized cells and that these bud primordia appeared to be arrested in their development. The fact that additional bud primordia were present within the explant suggests that further manipulation of the explant is helpful to maximize the potential of this system.
ISSN:0918-9440
1618-0860
DOI:10.1007/s10265-004-0180-4