Streptococcus pneumoniae Forms Surface-Attached Communities in the Middle Ear of Experimentally Infected Chinchillas

• Published in: The Journal of infectious diseases 2009-03, Vol.199 (6), p.786-794
• Main Authors: Reid, Sean D., Hong, Wenzhou, Dew, Kristin E., Winn, Dana R., Pang, Bing, Watt, James, Glover, David T., Hollingshead, Susan K., Swords, W. Edward
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Bacteria; Bacteriology; Biofilms; Biological and medical sciences; Cell Survival; Chinchilla; Disease Models, Animal; Ear, Middle - microbiology; Ear, Middle - pathology; Ear, Middle - ultrastructure; Ears; Fundamental and applied biological sciences. Psychology; Infections; Infectious diseases; Matrix materials; Medical sciences; Microbiology; Microscopy, Electron, Scanning; Middle ear; Miscellaneous; Neutrophils; Otitis media; Pneumonia, Pneumococcal - transmission; Rodentia; Streptococcus pneumoniae; Streptococcus pneumoniae - growth & development; Streptococcus pneumoniae - ultrastructure
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/597042

BackgroundStreptococcus pneumoniae (pneumococcus) causes respiratory and systemic infections that are a major public health problem worldwide. It has been postulated that pneumococci persist in vivo in biofilm communities MethodsIn this study, we analyzed whether pneumococci form biofilms in vivo, and if so, whether biofilms correlated with bacterial persistence. Chinchillas were infected with S. pneumoniae TIGR4 and euthanized at varying times after infection, after which the superior ear bullae were excised and examined by culture and microscopy ResultsDense material, resembling the biofilms of other otitis media pathogens, was visible in the middle ear as late as 12 days after infection. Scanning electron microscopy revealed bacteria within an electron-dense matrix, similar to pneumococcal biofilms formed in vitro. Viability staining revealed groups of viable diplococci, as well as viable and nonviable host cells, attached to a fibrous matrix that was positive when stained with propidium iodide. Cryosections of biofilms were treated with polyclonal antibodies against the pneumococcal surface components pneumococcal surface protein A family 2, pneumococcal surface protein C, choline-binding protein, and neuraminidase, coupled with appropriate secondary antibody conjugates. Immunofluorescent staining showed the presence of pneumococcal communities within the material recovered from the middle ear chamber ConclusionsOn the basis of these data, we conclude that pneumococci form biofilms in vivo and that this process may be intertwined with the formation of neutrophil extracellular traps. These findings provide new insights into the potential causes of antibiotic treatment failure and bacterial persistence in chronic pneumococcal otitis media