Characterization of rhesus macaque natural killer activity against a rhesus-derived target cell line at the single-cell level

Natural killer (NK) cells and NK cell activities in the rhesus macaque have been incompletely characterized. Using a recently developed rhesus NK target cell line with down-regulated MHC-I (B116 Lo) as stimulators and FACS-sorted cells as effectors in a 4-h [ 51Cr]-release assay we showed that the C...

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Bibliographic Details
Published in:Cellular immunology 2004-09, Vol.231 (1), p.85-95
Main Authors: Andersen, Hanne, Rossio, Jeffrey L., Coalter, Vicky, Poore, Barbara, Martin, Maureen P., Carrington, Mary, Lifson, Jeffrey D.
Format: Article
Language:English
Subjects:
AIDS
Animals
CD3 Complex - metabolism
CD8 Antigens - metabolism
Cells, Cultured
Cytokines - metabolism
Cytotoxicity, Immunologic
Degranulation
Down-Regulation
Histocompatibility Antigens Class I - immunology
Histocompatibility Antigens Class I - metabolism
Humans
Intracellular cytokine
Killer Cells, Natural - immunology
Macaca mulatta - immunology
Male
MHC
NK cells
Rhesus macaque
Viral
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Summary:Natural killer (NK) cells and NK cell activities in the rhesus macaque have been incompletely characterized. Using a recently developed rhesus NK target cell line with down-regulated MHC-I (B116 Lo) as stimulators and FACS-sorted cells as effectors in a 4-h [ 51Cr]-release assay we showed that the CD3 −CD8 lo subpopulation is the primary effector population for NK cell-mediated cytolysis. The majority of these cells co-express CD16, CD11b, NKG2D, and NKp46. To evaluate functional activity at the individual cell level, we employed intracellular cytokine staining and a flow cytometric assay for degranulation, based on cell surface CD107a expression. Flow cytometric analysis revealed that a greater proportion of NK cells degranulated than produced cytokines in response to B116 Lo stimulation; the frequency of CD107a-expressing cells within the total NK cell population ranging from 5 to 39%. Somewhat surprisingly, we did not find a significant correlation between lysis, measured by [ 51Cr]-release assay, and the size of the degranulating NK cell population, implying that additional mechanisms may regulate lytic activity. Use of these approaches should facilitate an improved understanding of NK activity in the rhesus macaque.
ISSN:0008-8749
1090-2163
DOI:10.1016/j.cellimm.2004.12.004