Highly dense protein layers confirmed by atomic force microscopy and quartz crystal microbalance

Protein adsorption on a gold surface is investigated by comparing the results of quartz crystal microbalance method and atomic force microscopy. The adsorption of streptavidin on functional gold surfaces is directly monitored by a quartz crystal microbalance, and confirmed by atomic force microscopy...

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Bibliographic Details
Published in:Journal of Bioscience and Bioengineering 2004, Vol.97 (2), p.138-140
Main Authors: Kim, Jongmin, Yamasaki, Ryujiroh, Park, Jongwan, Jung, Hosup, Lee, Heayeon, Kawai, Tomoji
Format: Article
Language:English
Subjects:
ADSORPTION
atomic force microscopy (AFM)
BALANCES
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
MICROSCOPY
protein film
PROTEINS
QUARTZ
quartz crystal microbalance (QCM)
streptavidin
STREPTOMYCES
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Summary:Protein adsorption on a gold surface is investigated by comparing the results of quartz crystal microbalance method and atomic force microscopy. The adsorption of streptavidin on functional gold surfaces is directly monitored by a quartz crystal microbalance, and confirmed by atomic force microscopy. For this investigation, a modified gold substrate is fabricated to obtain a topographic image of streptavidin molecules. Both methods show a correlation in terms of the highly dense protein single-layer formation, and the modified gold electrode shows a slightly denser protein layer formation because of the difference in substrate geometry as compared with that of a mica surface.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(04)70182-6