Pituitary adenylate cyclase-activating polypeptide promotes differentiation of mouse neural stem cells into astrocytes

We have found that pituitary adenylate cyclase-activating polypeptide (PACAP) employed at the physiological concentrations induces the differentiation of mouse neural stem cells into astrocytes. The differentiation process was not affected by cAMP analogues such as dibutylic cAMP (db-cAMP) or 8Br-cA...

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Bibliographic Details
Published in:Regulatory peptides 2005-03, Vol.126 (1), p.115-122
Main Authors: Ohno, Fusako, Watanabe, Jun, Sekihara, Hohkai, Hirabayashi, Takahiro, Arata, Satoru, Kikuyama, Sakae, Shioda, Seiji, Nakaya, Kazuyasu, Nakajo, Shigeo
Format: Article
Language:English
Subjects:
Animals
Astrocytes - physiology
Biological and medical sciences
Cell Differentiation - drug effects
Cell Differentiation - physiology
Cells, Cultured
Differentiation
Fundamental and applied biological sciences. Psychology
Mice
Nerve Growth Factors - pharmacology
Nerve Tissue - cytology
Nerve Tissue - physiology
Neural stem cell
Neuropeptides - pharmacology
Neurotransmitter Agents - pharmacology
PAC1
PACAP receptor
Pituitary Adenylate Cyclase-Activating Polypeptide
Pituitary adenylate cyclase-activating polypeptide (PACAP)
Stem Cells - physiology
Vertebrates: endocrinology
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Summary:We have found that pituitary adenylate cyclase-activating polypeptide (PACAP) employed at the physiological concentrations induces the differentiation of mouse neural stem cells into astrocytes. The differentiation process was not affected by cAMP analogues such as dibutylic cAMP (db-cAMP) or 8Br-cAMP or by the specific competitive inhibitor of protein kinase A, Rp-adenosine-3′,5′-cyclic monophosphothioate triethylamine salt (Rp-cAMP). Expression of the PACAP receptor (PAC1) in neural stem cells was detected by both RT–PCR and immunoblot using an affinity-purified antibody. The PACAP selective antagonist, PACAP 6-38, had an inhibitory effect on the PACAP-induced differentiation of neural stem cells into astrocytes. These results indicate that PACAP acts on the PAC1 receptor on the plasma membrane of mouse neural stem cells, with the signal then transmitted intracellularly via a PAC1-coupled G protein, does not involve Gs. This signaling mechanism may thus play a crucial role in the differentiation of neural stem cells into astrocytes.
ISSN:0167-0115
1873-1686
DOI:10.1016/j.regpep.2004.08.028