Impact of N-Terminal Domains for Corticotropin-Releasing Factor (CRF) Receptor−Ligand Interactions

• Published in: Biochemistry (Easton) 2005-02, Vol.44 (5), p.1614-1623
• Main Authors: Klose, Jana, Fechner, Klaus, Beyermann, Michael, Krause, Eberhard, Wendt, Norbert, Bienert, Michael, Rudolph, Rainer, Rothemund, Sven
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Circular Dichroism; Corticotropin-Releasing Hormone - chemistry; Corticotropin-Releasing Hormone - metabolism; Disulfides - chemistry; Humans; Ligands; Mice; Molecular Sequence Data; Molecular Weight; Peptide Fragments - biosynthesis; Peptide Fragments - chemistry; Peptide Fragments - genetics; Peptide Fragments - metabolism; Protein Binding; Protein Folding; Protein Structure, Tertiary; Rats; Receptors, Corticotropin-Releasing Hormone - biosynthesis; Receptors, Corticotropin-Releasing Hormone - chemistry; Receptors, Corticotropin-Releasing Hormone - genetics; Receptors, Corticotropin-Releasing Hormone - metabolism; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Recombinant Proteins - isolation & purification; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Urocortins
• ISSN: 0006-2960; 1520-4995
• DOI: 10.1021/bi049022e

The large extracellular N-terminal domains (NTs) of class B G protein-coupled receptors serve as major ligand binding sites. However, little is known about the ligand requirements for interactions with these receptor domains. Recently, we have shown that the most potent CRF receptor agonist urocortin 1 (Ucn1) has two segregated receptor binding sites Ucn1(1−21) and Ucn1(32−40). For locating the receptor domains interacting with these two sites, we have investigated the binding of appropriate Ucn1 analogues to the receptor N-termini compared to the corresponding full-length receptors. For this purpose receptor NTs of CRF(rat) subtypes 1 and 2(α) without their signal sequences were overexpressed in Escherichia coli and folded in vitro. For CRF2(a)-rNT, which bears five cysteine residues (C2−C6), the disulfide arrangement C2−C5 and C4−C6 was found, leaving C3 free. This is consistent with the disulfide pattern of CRF1-rNT, which has six cysteines and in which C1 is paired with C3. Binding studies of N-terminally truncated or C-terminally modified Ucn1 analogues demonstrate that it is the C-terminal part, Ucn1(11−40), that binds to receptor NT, indicating a two-domain binding mechanism for Ucn binding to receptor NT. Since the binding of Ucn1 to the juxtamembrane domain has been shown to be segregated from binding to the receptor N-terminus [Hoare et al. (2004) Biochemistry 43, 3996−4011], a third binding domain should exist, probably comprising residues 8−10 of Ucn, which particularly contribute to a high-affinity binding to full-length receptors but not to receptor NT.