In vitro release of growth factors from platelet-rich fibrin (PRF): a proposal to optimize the clinical applications of PRF

• Published in: Oral surgery, oral medicine, oral pathology, oral radiology and endodontics oral medicine, oral pathology, oral radiology and endodontics, 2009-07, Vol.108 (1), p.56-61
• Main Authors: Su, Chen Yao, DDS, PhD, Kuo, Ya Po, MS, Tseng, Yu Hong, DDS, Su, Ching-Hua, PhD, Burnouf, Thierry, PhD
• Format: Article
• Language: English
• Subjects: Blood Coagulation - physiology; Blood Platelets - physiology; Dentistry; Electrophoresis, Polyacrylamide Gel; Epidermal Growth Factor - analysis; Epidermal Growth Factor - secretion; Erythrocyte Count; Fibrin - physiology; Humans; Insulin-Like Growth Factor I - analysis; Insulin-Like Growth Factor I - secretion; Intercellular Signaling Peptides and Proteins - analysis; Intercellular Signaling Peptides and Proteins - secretion; Leukocyte Count; Platelet Count; Platelet-Derived Growth Factor - analysis; Platelet-Derived Growth Factor - secretion; Serum - physiology; Surgery; Transforming Growth Factor beta1 - analysis; Transforming Growth Factor beta1 - secretion; Vascular Endothelial Growth Factor A - analysis; Vascular Endothelial Growth Factor A - secretion
• ISSN: 1079-2104; 1528-395X
• DOI: 10.1016/j.tripleo.2009.02.004

Objective Determine the release of growth factors (GF) from platelet-rich fibrin (PRF) and supernatant serum to optimize clinical use. Study design Platelet-derived growth factors-AB (PDGF-AB), transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), epidermal growth factor (EGF), and insulin-like growth factor-1 (IGF-1) were quantified in PRF releasate and in the supernatant serum (N = 8) over 300 minutes after clot formation. Protein profiles were determined by SDS-PAGE. Results Mean quantity of PDGF-AB, TGF-ß1, VEGF, and EGF in PRF releasate increased significantly to about 52, 72, 1, and 3 ng, respectively, whereas mean IGF-1 content remained at 250 ng. GF was also found in serum supernatant. Protein profiles of the releasates and the supernatant serum were similar. Conclusion The PRF membrane should be used immediately after formation to maximize release of GF to the surgical site. The remaining fluid can be recovered as an additional source of GF for grafting.