ITS-RFLP- and RAPD-based genetic variability of Trypanosoma cruzi I, human and vector strains in Santander, Colombia

Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller...

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Bibliographic Details
Published in:Parasitology research (1987) 2009-08, Vol.105 (2), p.519-528
Main Authors: Luna-Marín, Katherine Paola, Jaramillo-Londoño, Claudia Lorena, Hernández-Torres, Jorge, Gutiérrez-Marín, Reynaldo, Vallejo, Gustavo Adolfo, Angulo-Silva, Víctor Manuel
Format: Article
Language:English
Subjects:
Animals
Chagas Disease - parasitology
Cluster Analysis
Colombia
DNA Fingerprinting - methods
DNA, Protozoan - genetics
DNA, Ribosomal Spacer - genetics
Genotype
Humans
Molecular Epidemiology
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
Triatominae - parasitology
Trypanosoma cruzi - classification
Trypanosoma cruzi - genetics
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Summary:Chagas disease is a severe public health problem in Latin-American countries. In Colombia, the predominance of Trypanosoma cruzi I has been described in the literature, with a broad heterogeneity between strains. However, most of the studies carried out centered on isoenzyme analysis, with a smaller number that focus on other molecular methods. In this report, we discuss the results of a molecular analysis of T. cruzi I strains, isolated from the domestic cycle, from the department of Santander, one of the territorial divisions where the prevalence of infection is highest. Internal transcribed spacer-restriction fragment length polymorphism and random amplification of polymorphic DNA were used to characterize 16 strains from human and vector (Triatominae) hosts. The data reveal a clustering based on the biological origin. Human and vector strains grouped separately; however, three vector strains clustered together with human strains. These results indicate that genetic differences exist in the strains that infect both hosts. We conclude that T. cruzi I populations in the domestic cycle of transmission of Chagas disease in Santander are heterogeneous and are composed of different clones. The epidemiological and biological implications are discussed.
ISSN:1432-1955
DOI:10.1007/s00436-009-1422-0