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Proteomic and transcriptomic characterization of interferon-α-induced human primary T helper cells

Interferon‐α (IFN‐α) is a multifunctional cytokine that modulates immune response. In spite of the numerous comprehensive studies on the effects of IFN‐α on various cell types, novel characteristics of this versatile agent emerge continuously. In the present study a differential proteomic approach w...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2005-02, Vol.5 (2), p.371-379
Main Authors: Rosengren, Arsi T., Nyman, Tuula A., Syyrakki, Saija, Matikainen, Sampsa, Lahesmaa, Riitta
Format: Article
Language:English
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Summary:Interferon‐α (IFN‐α) is a multifunctional cytokine that modulates immune response. In spite of the numerous comprehensive studies on the effects of IFN‐α on various cell types, novel characteristics of this versatile agent emerge continuously. In the present study a differential proteomic approach was used to identify new IFN‐α‐regulated proteins in human primary CD4+ T cells. Two IFN‐α‐inducible proteins, soluble N‐ethylmaleimide‐sensitive factor attachment protein &α; (&α;‐SNAP) and cleavage stimulation factor‐64 (CstF‐64) previously not described in this context, were identified. Additionally, several proteins already known as IFN‐stimulated genes were observed. The results of proteomics experiments were further studied at the mRNA level using real‐time reverse transcriptase‐polymerase chain reaction (RT‐PCR). Both peripheral blood and cord blood CD4+ T cells were used in order to see if there are differences in IFN‐α response between these populations. Differences were observed between the IFN‐α‐induced expression kinetics in peripheral blood and cord blood transcripts. The induction was more rapid in peripheral blood than in cord blood cells. CstF‐64 expression was upregulated by IFN‐α at the protein, but not at the mRNA level.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200400967