Quantitative determination of vigabatrin and gabapentin in human serum by gas chromatography–mass spectrometry

Published methods for routine clinical monitoring of vigabatrin and gabapentin are often very laborious. A simple GC-MS method was developed for the simultaneous quantitative determination of vigabatrin and gabapentin in human serum. After protein precipitation, the compounds are derivatized by meth...

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Bibliographic Details
Published in:Clinica chimica acta 2005-04, Vol.354 (1), p.147-151
Main Authors: Borrey, Daniëlle C.R., Godderis, Kristof O., Engelrelst, Veronique I.L., Bernard, Dirk R., Langlois, Michel R.
Format: Article
Language:English
Subjects:
Amines - blood
Anti-epileptics
Calibration
Cyclohexanecarboxylic Acids - blood
Gabapentin
gamma-Aminobutyric Acid - blood
Gas Chromatography-Mass Spectrometry - methods
GC-MS
Humans
Reproducibility of Results
Sensitivity and Specificity
Vigabatrin
Vigabatrin - blood
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Summary:Published methods for routine clinical monitoring of vigabatrin and gabapentin are often very laborious. A simple GC-MS method was developed for the simultaneous quantitative determination of vigabatrin and gabapentin in human serum. After protein precipitation, the compounds are derivatized by methylation and analysed on a polydimethylsiloxane column using splitless injection. Cyclobarbital is used as the internal standard. To attain maximal sensitivity, detection is performed in selected ion monitoring mode. The method was fully validated and linear calibration curves were obtained in the concentration ranges from 5 to 80 μg/mL for vigabatrin and from 5 to 30 μg/mL for gabapentin. The within-day and day-to-day relative standard deviations at three different concentration levels were
ISSN:0009-8981
1873-3492
DOI:10.1016/j.cccn.2004.11.023