Investigation of artificial biomembrane systems in biopartitioning micellar chromatography by method of mathematical design

An attempt to create and study an artificial membrane system was realized via biopartitioning micellar liquid chromatography. Towards this end the known formula of membrane permeability (on the basis of Fick's diffusion equation) was modified so that membrane permeability may be estimated in term...

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Bibliographic Details
Published in:Biomedical chromatography 2005-03, Vol.19 (2), p.169-177
Main Authors: Rukhadze, Marina D., Bezarashvili, George S., Kutkhashvili, Maya G., Sigua, Kethevan I.
Format: Article
Language:English
Subjects:
Anions
artificial membranes
Barbital - chemistry
Benzoic Acid - chemistry
biopartitioning micellar chromatography
Carbamazepine - chemistry
Cations
Chemical Phenomena
Chemistry, Physical
Chromatography - methods
Diffusion
Dihydroxyphenylalanine - chemistry
Fick's diffusion equation
Hydrophobic and Hydrophilic Interactions
Mathematics
membrane permeability
Membranes, Artificial
Micelles
Pentobarbital - analogs & derivatives
Pentobarbital - chemistry
Permeability
Phenylalanine - chemistry
Polyethylene Glycols
Riboflavin - chemistry
Saccharin - chemistry
Surface-Active Agents
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Summary:An attempt to create and study an artificial membrane system was realized via biopartitioning micellar liquid chromatography. Towards this end the known formula of membrane permeability (on the basis of Fick's diffusion equation) was modified so that membrane permeability may be estimated in terms of chromatographic characteristics. The two‐factoral experiments on the basis of mathematical design of second order were carried out. The regression equations are derived which describe the dependence of membrane permeability on the concentration of polyoxyethylene (23) lauryl ether in the mobile phase and its flow‐rate for compounds with biomedical significance. Some regularities were revealed, which characterize the permeability of compounds of the different nature through membranes. The extremal dependence (with passing through minimum) of permeability on the concentration of non‐ionic surfactant was observed for anionic compounds. The increasing character of permeability in relation with flow‐rate of mobile phase was recognized for cationic samples. Both dependences were basically fulfilled for zwitterionic compounds. Copyright © 2005 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.469