Replication-coupled modulation of early replicating chromatin domains detected by anti-actin antibody

Evidence is presented for the reversible, cold‐dependent immunofluorescence detection of the epitope (hereafter referred to as epiC), recognized by a monoclonal anti‐actin antibody in diploid human fibroblast cell nuclei and mitotic chromosomes. The nuclear/chromosomal epiC was detected in a cell cy...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2005-04, Vol.94 (5), p.899-916
Main Authors: Fidlerová, Helena, Mašata, Martin, Malínský, Jan, Fialová, Markéta, Cvac̆ková, Zuzana, Louz̆ecká, Alena, Koberna, Karel, Berezney, Ronald, Ras̆ka, Ivan
Format: Article
Language:English
Subjects:
Actins - immunology
anti-actin
Blotting, Western
cell cycle
Cells, Cultured
chromatin
Chromatin - chemistry
cold-dependent epitope detection
DNA Replication
G1 Phase
Humans
Image Processing, Computer-Assisted
immunofluorescence microscopy
Microscopy, Fluorescence
nuclear actin
S Phase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Evidence is presented for the reversible, cold‐dependent immunofluorescence detection of the epitope (hereafter referred to as epiC), recognized by a monoclonal anti‐actin antibody in diploid human fibroblast cell nuclei and mitotic chromosomes. The nuclear/chromosomal epiC was detected in a cell cycle window beginning in early S phase and extending through S phase, G2 phase, mitosis until early G1 phase of the subsequent daughter cells. A small but significant level of co‐localization was measured between the nuclear epiC and active sites of DNA replication in early S phase. The level of co‐localization was strikingly enhanced beginning approximately 1 h after the initial labeling of early S phase replicating chromatin domains. In contrast, epiC did not co‐localize with late S phase replicated chromatin either during DNA replication or at any other time in the cell cycle. We propose a replication‐coupled modulation of early S phase replicated chromatin domains that is detected by the chromatin epiC positivity, persists on the chromatin domains from early S until early G1 of the next cell generation, and may be involved in the regulation and/or coordination of replicational and transcriptional processes during the cell cycle. Further studies will be required to resolve the possible role of nuclear actin in this modulation process. J. Cell. Biochem. 94: 899–916, 2005. © 2005 Wiley‐Liss, Inc.
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.20374