Plasmacytoid Dendritic Cells Control TLR7 Sensitivity of Naive B Cells via Type I IFN

Detailed information of human B cell activation via TLR may lead to a better understanding of B cell involvement in autoimmunity and malignancy. In this study we identified a fundamental difference in the regulation of TLR7- and TLR9-mediated B cell stimulation: whereas the induction of polyclonal n...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2005-04, Vol.174 (7), p.4043-4050
Main Authors: Bekeredjian-Ding, Isabelle Beatrice, Wagner, Moritz, Hornung, Veit, Giese, Thomas, Schnurr, Max, Endres, Stefan, Hartmann, Gunther
Format: Article
Language:English
Subjects:
B-Lymphocytes - cytology
B-Lymphocytes - physiology
Blood Cells
Cell Differentiation
Cell Proliferation
Dendritic Cells - metabolism
Dendritic Cells - physiology
Humans
Interferon Type I - metabolism
Interferon Type I - physiology
Ligands
Lymphocyte Activation
Membrane Glycoproteins - genetics
Membrane Glycoproteins - physiology
Receptors, Cell Surface - genetics
Receptors, Cell Surface - physiology
Toll-Like Receptor 2
Toll-Like Receptor 4
Toll-Like Receptor 7
Toll-Like Receptor 9
Toll-Like Receptors
Up-Regulation - genetics
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Summary:Detailed information of human B cell activation via TLR may lead to a better understanding of B cell involvement in autoimmunity and malignancy. In this study we identified a fundamental difference in the regulation of TLR7- and TLR9-mediated B cell stimulation: whereas the induction of polyclonal naive B cell proliferation by the TLR7 ligands resiquimod (R848) and loxoribine required the presence of plasmacytoid dendritic cells (PDCs), activation via the TLR9 ligand CpG was independent of PDCs. We found that PDC-derived type I IFN enhanced TLR7 sensitivity of B cells by selectively up-regulating TLR7 expression. In contrast the expression levels of TLR9 and of other TLRs studied remained unchanged. In the presence of type I IFN, TLR7 ligation triggered polyclonal B cell expansion and B cell differentiation toward Ig-producing plasma cells; notably, this occurred independently of T cell help and B cell Ag. Human B cells did not respond to ligands of other TLRs including TLR2, TLR4 and TLR6 with and without type I IFN. In conclusion, our results reveal a distinct regulation of TLR7 and TLR9 function in human B cells and highlight TLR7 and TLR9 as unique targets for therapeutic intervention in B cell-mediated immunity and disease.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.174.7.4043