Assessing interethnic admixture using an X-linked insertion-deletion multiplex

• Published in: American journal of human biology 2009-09, Vol.21 (5), p.707-709
• Main Authors: Ribeiro-Rodrigues, Elzemar Martins, dos Santos, Ney Pereira Carneiro, dos Santos, Ândrea Kely Campos Ribeiro, Pereira, Rui, Amorim, António, Gusmão, Leonor, Zago, Marco Antonio, dos Santos, Sidney Emanuel Batista
• Format: Article
• Language: English
• Subjects: American Native Continental Ancestry Group - genetics; Chromosomes, Human, X - genetics; Chromosomes, Human, Y - genetics; Continental Population Groups - genetics; Humans; INDEL Mutation; Indians, Central American - genetics; Indians, North American - genetics; Indians, South American - genetics; Polymerase Chain Reaction
• ISSN: 1042-0533; 1520-6300
• DOI: 10.1002/ajhb.20950

In this study, a PCR multiplex was optimized, allowing the simultaneous analysis of 13 X‐chromosome Insertion/deletion polymorphisms (INDELs). Genetic variation observed in Africans, Europeans, and Native Americans reveals high inter‐population variability. The estimated proportions of X‐chromosomes in an admixed population from the Brazilian Amazon region show a predominant Amerindian contribution (≅41%), followed by European (≅32%) and African (≅27%) contributions. The proportion of Amerindian contribution based on X‐linked data is similar to the expected value based on mtDNA and Y‐chromosome information. The accuracy for assessing interethnic admixture, and the high differentiation between African, European, and Native American populations, demonstrates the suitability of this INDEL set to measure ancestry proportions in three‐hybrid populations, as it is the case of Latin American populations. Am. J. Hum. Biol. 2009. © 2009 Wiley‐Liss, Inc.