Identification of a New Spore Wall Protein from Encephalitozoon cuniculi

Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously id...

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Bibliographic Details
Published in:Infection and Immunity 2006, Vol.74 (1), p.239-247
Main Authors: Xu, Yanji, Takvorian, Peter, Cali, Ann, Wang, Fang, Zhang, Hong, Orr, George, Weiss, Louis M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antigens, Protozoan - biosynthesis
Antigens, Protozoan - isolation & purification
Antigens, Protozoan - metabolism
Biological and medical sciences
Cloning, Molecular
Computational Biology - methods
Encephalitozoon cuniculi
Encephalitozoon cuniculi - chemistry
Encephalitozoon cuniculi - metabolism
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Fungal and Parasitic Infections
Fungal Proteins - biosynthesis
Fungal Proteins - isolation & purification
Fungal Proteins - metabolism
Glycoproteins - biosynthesis
Glycoproteins - isolation & purification
Glycoproteins - metabolism
Glycosylation
Immunohistochemistry
Mannose - metabolism
Microbiology
Microscopy, Fluorescence
Microscopy, Immunoelectron
Microsporidia
Molecular Sequence Data
Spores, Fungal - chemistry
Spores, Fungal - metabolism
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Summary:Microsporidia form environmentally resistant spores that are critical for their host-to-host transmission and persistence in the environment. The spore walls of these organisms are composed of two layers, the exospore and the endospore. Two spore wall proteins (SWP1 and SWP2) have been previously identified in members of the Encephalitozoonidae family. These proteins localize to the exospore. The endospore is known to contain chitin, and a putative glycosylphosphatidylinositol (GPI)-anchored chitin deacetylase has been localized to the plasmalemma-endospore interface. Using proteomic techniques, we have identified a new spore wall protein (SWP3) that is located in the endospore. The gene for this protein is located on chromosome 1 and corresponds to the open reading frame ECU01_1270. SWP3 is predicted to have a signal peptide and to be GPI anchored. Consistent with these modifications, two-dimensional electrophoresis demonstrated that SWP3 has an acidic pI and a molecular mass of
ISSN:0019-9567
1098-5522
DOI:10.1128/IAI.74.1.239-247.2006