In vitro effect of vitamin E on lectin-stimulated porcine peripheral blood mononuclear cells

In order to analyze the effect of vitamin E on Th1 and Th2 cytokine production, porcine peripheral blood mononuclear cells (PBMC) were isolated from healthy pigs ( n = 8) and cultured with either 0, 10, 50, or 100 μM of vitamin E (α-tocopherol). PBMC were stimulated with PHA for either, 24 h to dete...

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Bibliographic Details
Published in:Veterinary immunology and immunopathology 2009-09, Vol.131 (1), p.9-16
Main Authors: Hernández, Jesús, Soto-Canevett, Eneida, Pinelli-Saavedra, Araceli, Resendiz, Mónica, Moya-Camarena, Silvia Y., Klasing, Kirk C.
Format: Article
Language:English
Subjects:
Animals
blood
Cytokines
Cytokines - biosynthesis
GATA3
GATA3 Transcription Factor - genetics
immunomodulators
in vitro studies
lectins
Lymphocyte Activation - drug effects
monocytes
Phytohemagglutinins - pharmacology
RNA, Messenger - analysis
Swine
T-bet
T-Box Domain Proteins - genetics
TBX21
Th1 Cells - immunology
Th2 Cells - immunology
Vitamin E
Vitamin E - pharmacokinetics
Vitamin E - pharmacology
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Summary:In order to analyze the effect of vitamin E on Th1 and Th2 cytokine production, porcine peripheral blood mononuclear cells (PBMC) were isolated from healthy pigs ( n = 8) and cultured with either 0, 10, 50, or 100 μM of vitamin E (α-tocopherol). PBMC were stimulated with PHA for either, 24 h to determine: (a) the concentration of tocopherol incorporated into the cell membrane, (b) cytokine production and (c) Th1 and Th2 regulators gene expression; or 72 h to determine the proliferation of PBMC. Vitamin E was incorporated into the PBMC in a dose dependent manner, giving as a result a high proliferation of cells irrespective of the dose of vitamin E used. Regarding cytokine production, vitamin E consistently decreases the mRNA expression and the percentage of cells producing IL-10. Vitamin E did not influence the production of IFN-γ but the lowest level of vitamin E (10 μM) was sufficient to maximally increase the proportion of cells producing IL-2, to diminish IL-4, and discreetly increase the mRNA expression of TBX21 vs. GATA3. In conclusion, our results revealed that vitamin E is able to suppress IL-10 production and to influence the production of IL-2, IL-4, and maybe TBX21. Vitamin E clearly has immunomodulatory effects, though further work in vivo to determine the physiological nature of these effects is warranted.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2009.03.001