Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS

An LC/MS/MS assay for the determination of tenofovir (TNF) was developed and validated for use with the EDTA anticoagulated human plasma matrix. Heparin-treated plasma and serum matrices were also validated. After addition of adefovir as an internal standard, trifluoroacetic acid was used to produce...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-01, Vol.830 (1), p.6-12
Main Authors: Delahunty, Tom, Bushman, Lane, Fletcher, Courtney V.
Format: Article
Language:English
Subjects:
Adefovir (PMEA)
Adenine - analogs & derivatives
Adenine - blood
Adenine - pharmacokinetics
Analysis
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Calibration
Chromatography, High Pressure Liquid - methods
Fundamental and applied biological sciences. Psychology
General pharmacology
HIV Infections - blood
Humans
LC/MS/MS
Mass Spectrometry - methods
Medical sciences
Organophosphonates - blood
Organophosphonates - pharmacokinetics
Pharmacology. Drug treatments
Plasma
Reference Standards
Reverse Transcriptase Inhibitors - blood
Reverse Transcriptase Inhibitors - pharmacokinetics
Sensitivity and Specificity
Tenofovir
Tenofovir (TNF)
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:An LC/MS/MS assay for the determination of tenofovir (TNF) was developed and validated for use with the EDTA anticoagulated human plasma matrix. Heparin-treated plasma and serum matrices were also validated. After addition of adefovir as an internal standard, trifluoroacetic acid was used to produce a protein-free extract. Chromatographic separation was achieved with a Polar-RP Synergi, 2.0 mm × 150 mm, reversed-phase analytical column. The mobile phase was 3% acetonitrile/1% acetic acid, aq. Detection of TNF and the internal standard was achieved by ESI MS/MS in the positive ion mode using 288/176 and 274/162 transitions, respectively. The method was linear from 10 to 750 ng/ml with a minimum quantifiable limit of 10 ng/ml when 250 μl aliquots were analyzed. The usefulness of this LC/MS/MS method to routinely monitor plasma concentrations of TNF was demonstrated along with its ability to assist in the performance of pharmacokinetic studies.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2005.10.015