Comparative modeling and analysis of microfluidic and conventional DNA microarrays

A theoretical analysis was developed to predict molecular hybridization rates for microarrays where samples flow through microfluidic channels and for conventional microarrays where samples remain stationary during hybridization. The theory was validated by using a multiplexed microfluidic microarra...

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Bibliographic Details
Published in:Analytical biochemistry 2006-01, Vol.348 (2), p.284-293
Main Authors: Benn, James A., Hu, Jenny, Hogan, Bradley J., Fry, Rebecca C., Samson, Leona D., Thorsen, Todd
Format: Article
Language:English
Subjects:
Biochips
Diffusion
DNA
DNA - analysis
DNA Probes - chemistry
Hybridization
Kinetics
Mass transport
Microarray
Microfluidic Analytical Techniques
Models, Chemical
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis
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Summary:A theoretical analysis was developed to predict molecular hybridization rates for microarrays where samples flow through microfluidic channels and for conventional microarrays where samples remain stationary during hybridization. The theory was validated by using a multiplexed microfluidic microarray where eight samples were hybridized simultaneously against eight probes using 60-mer DNA strands. Mass transfer coefficients ranged over three orders of magnitude where either kinetic reaction rates or molecular diffusion rates controlled overall hybridization rates. Probes were printed using microfluidic channels and also conventional spotting techniques. Consistent with the theoretical model, the microfluidic microarray demonstrated the ability to print DNA probes in less than 1 min and to detect 10-pM target concentrations with hybridization times in less than 5 min.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2005.10.033