Development of a Transient Segregated Mathematical Model of the Semicontinuous Microbial Production Process of Dihydroxyacetone

• Published in: Biotechnology progress 2006, Vol.22 (1), p.278-284
• Main Authors: Bauer, Rüdiger, Hekmat, Dariusch
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Bioreactors; Biotechnology; Dihydroxyacetone - biosynthesis; Fundamental and applied biological sciences. Psychology; Gluconobacter oxydans - growth & development; Gluconobacter oxydans - metabolism; Kinetics; Models, Theoretical
• ISSN: 8756-7938; 1520-6033
• DOI: 10.1021/bp050342e

For the mathematical description of the semicontinuous two‐stage repeated‐fed‐batch fermentation of dihydroxyacetone (DHA), a novel segregated model incorporating transient growth rates was developed. The fermentation process was carried out in two stages. A viable, not irreversibly product‐inhibited culture was maintained in the first reactor stage until a predetermined DHA threshold value was reached. In the second reactor stage, high final product concentrations of up to 220 g L−1 were reached while the culture was irreversibly product‐inhibited. The experimentally observed changes of the physiological state of the culture due to product inhibition were taken into account by introducing a segregation into the mathematical model. It was shown that the state of the cells was dependent on the current environment and on the previous history. This phenomenon was considered in the model by utilizing delay time equations for the specific rates of growth on the primary and the secondary substrate. A comparison with reproducible measurements gave a good correlation between computation and experiment. The mathematical model was validated using independent own experimental data. A comparison with a stationary and nonsegregated model demonstrated the essential improvements of the novel model. It was deduced from the model calculations that high product formation rates of 3.3–3.5 g L−1 h−1 as well as high final DHA concentrations of 196–215 g L−1 can be obtained with a residual broth volume in the first reactor stage of 2% and a DHA threshold value in the range of 100–110g L−1.