The utilization of lipovitellin during blue crab ( Callinectes sapidus) embryogenesis

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2006-02, Vol.143 (2), p.201-208
• Main Authors: Walker, Anna, Ando, Seiichi, Smith, G. Denice, Lee, Richard F.
• Format: Article
• Language: English
• Subjects: Animals; Apoproteins - analysis; Blue crab; Brachyura - embryology; Brachyura - metabolism; Callinectes sapidus; Egg Proteins; Egg Proteins, Dietary - analysis; Egg Proteins, Dietary - metabolism; ELISA; Embryo, Nonmammalian - metabolism; Embryogenesis; Embryonic Development; Hepatopancreas; Immunoassay; Immunochemistry; Immunohistochemistry; Lipovitellin; Liver - metabolism; Marine; Yolk
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2005.11.015

Embryos of the blue crab Callinectes sapidus develop in egg sacs carried on the abdomen of the female. They develop over a period of 10–13 days at 28 °C and are nutritionally dependent on yolk until they emerge from the egg sacs as free-swimming zoeae. The principal component of blue crab yolk is lipovitellin (LpII), a water-soluble lipoprotein composed of approximately equal amounts of lipid and protein. We followed changes in the concentration of apoproteins of LpII during embryogenesis by ELISA and Western blots, using monoclonal antibodies against two LpII apoprotein associated peptides identified as Protein A (107 kDa) and Protein B (75 kDa). During embryogenesis there was a decrease in Protein B but an increase in two smaller peptides (52 and 35 kDa) that reacted with the Protein B antibody. Utilization of LpII during embryogenesis was also followed morphologically by immunohistochemistry. Utilization of LpII was slow in early embryonic stages, followed by rapid utilization in late embryonic stages, such that only traces of LpII were present at the end of embryogenesis. The cells of the developing hepatopancreas appear to play an important role in the utilization of LpII.