Protein nanoarray on Prolinker™ surface constructed by atomic force microscopy dip-pen nanolithography for analysis of protein interaction

Protein nanoarrays are addressable ensembles of nano‐scale protein domain on solid surfaces. This method can serve as a useful platform for ultraminiaturized bioanalysis. In this study, we investigated single molecular nanopatterning and molecular interaction of proteins that were immobilized on Pro...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2006-02, Vol.6 (4), p.1094-1103
Main Authors: Lee, Minsu, Kang, Dong-Ku, Yang, Hyun-Kyu, Park, Keun-Hyung, Choe, Soo Young, Kang, ChangSoo, Chang, Soo-Ik, Han, Moon Hi, Kang, In-Cheol
Format: Article
Language:English
Subjects:
Atomic force microscopy
Dip-pen nanolithography
Gold
Integrin alphaVbeta3 - chemistry
Integrin alphaVbeta3 - metabolism
Microscopy, Atomic Force - methods
Nanotechnology
Prolinker
Protein Array Analysis
Protein Binding
Protein nanoarray
Ribonuclease, Pancreatic - chemistry
Ribonuclease, Pancreatic - metabolism
Semiconductors
Single molecular interaction of protein
Surface Properties
Vitronectin - chemistry
Vitronectin - metabolism
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Summary:Protein nanoarrays are addressable ensembles of nano‐scale protein domain on solid surfaces. This method can serve as a useful platform for ultraminiaturized bioanalysis. In this study, we investigated single molecular nanopatterning and molecular interaction of proteins that were immobilized on Prolinker™ surface of gold‐coated silicon wafer by using dip‐pen nanolithography (DPN) method. Contact force and humidity were optimized at 0.01 nN and 80%, respectively. The domain features of protein nanoarrays were developed at the contact time of 5 s. The optimized conditions for the nanoarray process were applied to create protein nanoarray using integrin αvβ3 and angiogenin. Constructed protein nanoarrays using integrin αvβ3 have single molecular monolayer with regular domain shape (height 15 ± 5 nm). The changed height value due to the single molecular interaction between integrin αvβ3 and vitronectin was approximately 30 ± 5 nm on Prolinker™ surface as measured with atomic force microscopy tip. Taken together, these results suggest that protein nanoarray on Prolinker™ surface fabricated by well‐controlled DPN process can be used to analyze single molecular interaction of protein.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200500392