Hydrogen peroxide scavenging activity by non-steroidal anti-inflammatory drugs

• Published in: Life sciences (1973) 2005-04, Vol.76 (24), p.2841-2848
• Main Authors: Costa, David, Gomes, Ana, Reis, Salette, Lima, José L.F.C., Fernandes, Eduarda
• Format: Article
• Language: English
• Subjects: Acridines; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Catalase - chemistry; Free Radical Scavengers - chemistry; Hydrogen Peroxide - chemistry; Hydrogen peroxide scavenging activity; Luminescent Measurements; Microplate chemiluminescence assay; NSAIDs; Reactive Oxygen Species - chemistry
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2004.10.052

Hydrogen peroxide (H 2O 2) has been shown to be formed during inflammatory processes and is implicated in its pathophysiology. Thus, a putative scavenging activity against this reactive oxygen specie (ROS) by anti-inflammatory drugs may be of great therapeutical value. The present study was undertaken to evaluate the scavenging activity for H 2O 2 by several non-steroidal anti-inflammatory drugs (NSAIDs), namely indomethacin, acemetacin, etodolac, tolmetin, ketorolac, oxaprozin, sulindac and its metabolites sulindac sulfide and sulindac sulfone. The H 2O 2 scavenging assay was performed by measuring H 2O 2-elicited lucigenin chemiluminescence using a microplate reader. The specificity of the method was confirmed by the use of catalase, which completely prevented the H 2O 2-induced lucigenin chemiluminescence. The endogenous antioxidants melatonin and reduced glutathione (GSH) were used as positive controls. The obtained results demonstrated that all the studied NSAIDs display H 2O 2 scavenging activity, although in different extents. The ranking order of potency found was sulindac sulfone > sulindac sulfide > GSH > sulindac > indomethacin > acemetacin > etodolac > oxaprozin > ketorolac ≈ melatonin > tolmetin.