Human marrow mesenchymal stem cell culture: serum-free medium allows better expansion than classical α-MEM medium

:  The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10–20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for their clinical use. Therefore, we have evaluate...

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Bibliographic Details
Published in:European journal of haematology 2006-04, Vol.76 (4), p.309-316
Main Authors: Meuleman, Nathalie, Tondreau, Tatiana, Delforge, Alain, Dejeneffe, Marielle, Massy, Martine, Libertalis, Mark, Bron, Dominique, Lagneaux, Laurence
Format: Article
Language:English
Subjects:
Bone Marrow Cells - cytology
Bone Marrow Cells - physiology
Cell Culture Techniques - methods
cell therapy
Cells, Cultured
Culture Media, Serum-Free
expansion
Hematopoiesis - physiology
Humans
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - physiology
Organic Chemicals
serum-free medium
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Summary::  The expansion of mesenchymal stem cells (MSCs) strongly depends on the culture conditions and requires medium supplemented with 10–20% fetal calf serum (FCS) to generate relevant numbers of cells. However, the presence of FCS is a major obstacle for their clinical use. Therefore, we have evaluated the capacity of expansion of MSC in a commercial serum‐free medium (UC) supplemented with a serum substitute (ULTROSER®) in comparison with a classical medium α‐MEM containing 15% FBS. Bone marrow‐mononuclear cells collected from 12 volunteer healthy donors were expanded in two different culture media. MSCs isolated in the both media were morphologically similar and expressed identical phenotypic markers. After the primoculture (P0) and one passage, we obtained significantly more MSC and CFU‐F progenitors in UC medium than in αMEM. Their multipotentiality was preserved during culture, as well as their capacity to support haematopoiesis. In conclusion, our observations strongly suggest that UC is an optimal medium for ex vivo expansion of MSC: it allows a better cell expansion, preserves cell multipotentiality, reduces the culture period and contains low concentration of serum substitute. This medium seems suitable for clinical scale expansion of MSC.
ISSN:0902-4441
1600-0609
DOI:10.1111/j.1600-0609.2005.00611.x