A simple high performance liquid chromatography assay for monitoring plasma concentrations of tipranavir in HIV infected patients

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2006-03, Vol.832 (2), p.317-320
• Main Authors: Dailly, E., Reliquet, V., Victorri-Vigneau, C., Raffi, F., Jolliet, P.
• Format: Article
• Language: English
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; HIV Infections - blood; HIV Infections - drug therapy; Humans; Medical sciences; Pharmacology. Drug treatments; Pyridines - blood; Pyridines - therapeutic use; Pyrones - blood; Pyrones - therapeutic use; Reproducibility of Results; Reverse Transcriptase Inhibitors - blood; Reverse Transcriptase Inhibitors - therapeutic use; Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Tipranavir
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2006.02.004

A simple HPLC assay to determine plasma concentration of tipranavir is presented. A liquid/liquid extraction of the drugs in ethyl acetate/hexane from 250 μL of plasma is followed by a reversed phase isocratic HPLC assay with UV detection at 205 nm. The imprecision and inaccuracy are lower than 10%, the low limit of quantitation is 0.4 mg/L. Thus, this method can be used for therapeutic drug monitoring of tipranavir in HIV infected patients