Phospholipids from some common fungi associated with damp building materials

Methods for the analysis of fungal biomass in contaminated building materials have been limited to methods that use viable propagules as well as indicators of total biomass such as fungal glucan and ergosterol. Because of large differences in the survival times of spores, and limitations imposed by...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2006-02, Vol.384 (4), p.972-979, Article 972
Main Authors: Womiloju, Taiwo O, Miller, J. David, Mayer, Paul
Format: Article
Language:English
Subjects:
Air sampling
Biomass
Building materials
Chromatography, Liquid
Construction materials
Construction Materials - microbiology
Damp buildings
Ergosterol
Fungi
Fungi - chemistry
Fungi - classification
Fungi - isolation & purification
Glucan
Indicators
Lipids
liquid chromatography
mass spectrometry
Phospholipids
Phospholipids - analysis
Propagules
Qualitative analysis
Relative abundance
Spectrometry, Mass, Electrospray Ionization
Spores
Substrates
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Summary:Methods for the analysis of fungal biomass in contaminated building materials have been limited to methods that use viable propagules as well as indicators of total biomass such as fungal glucan and ergosterol. Because of large differences in the survival times of spores, and limitations imposed by the use of agar media to assess fungal cells for the former approach, no quantitative information and poor qualitative information is obtained. The use of the biochemical indicators provides reliable quantitative information but no qualitative data. Analysis of phospholipids of various bacteria and, recently, of fungi common in outdoor air has provided both qualitative and quantitative data on their distributions in various substrates including in outdoor air samples. In this report, we provide new data on the signature lipids of some fungi common on moldy building materials. Using the LC/MS/MS analysis described, an estimate can be made of the fresh weight of fungal cells and the relative abundance of the common genera of fungi typically found on moldy materials.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-005-0222-3