Surface Plasmon Resonance Imaging of Biomolecular Interactions on a Grating-Based Sensor Array

• Published in: Analytical chemistry (Washington) 2006-03, Vol.78 (6), p.2009-2018
• Main Authors: Singh, Bipin K, Hillier, Andrew C
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Biochemistry; Biosensors; Electrodes; Gold - chemistry; Proteins; Scientific apparatus & instruments; Sensitivity and Specificity; Serum Albumin, Bovine - chemistry; Sulfhydryl Compounds - chemistry; Surface Plasmon Resonance - methods; Surface Properties
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/ac0519209

A surface plasmon resonance sensor array based upon a grating substrate was developed for the detection of biomolecular interactions. The substrate consisted of a gold grating prepared by wet chemical treatment of a commercial recordable compact disk. A custom-built floating pin microspotter was constructed to deliver solutions containing ω-functionalized linear alkanethiols to the grating surface and produce an array of sensor elements with different exposed functional end groups. This array platform can be used to study biomolecular interactions in a label-free, sensitive, and high-throughput format. To illustrate the performance of this device, a test protein (bovine serum albumin) was exposed to sensor elements containing an array of functionalized alkanethiols possessing either activated carboxylic acid-, amine-, or hydroxyl-terminated regions. Local changes in plasmon resonance were monitored in a fixed-angle imaging configuration. Plasmon images clearly distinguish the degree of protein attachment at the various surfaces. The molecular binding events on the grating were also confirmed by ellipsometry. This grating-based SPR imaging platform represents a simple and robust method for performing label-free, high-sensitivity, and high-throughput detection of biomolecular interactions.