Tonic and Phasic Respiratory Drives to Human Genioglossus Motoneurons During Breathing

• Published in: Journal of neurophysiology 2006-04, Vol.95 (4), p.2213-2221
• Main Authors: Saboisky, Julian P, Butler, Jane E, Fogel, Robert B, Taylor, Janet L, Trinder, John A, White, David P, Gandevia, Simon C
• Format: Article
• Language: English
• Subjects: Action Potentials; Adult; Electromyography; Exhalation - physiology; Female; Humans; Hypoglossal Nerve - physiology; Inhalation - physiology; Lung Volume Measurements; Male; Middle Aged; Motor Neurons - physiology; Respiration; Respiratory Physiological Phenomena; Time Factors; Tongue - innervation; Tongue - physiology
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.00940.2005

1 Prince of Wales Medical Research Institute, Randwick and University of New South Wales, Sydney; 2 Department of Psychology, University of Melbourne, Victoria, Australia; and 3 Division of Sleep Medicine, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts Submitted 7 September 2005; accepted in final form 21 November 2005 A tongue muscle, the genioglossus (GG), is important in maintaining pharyngeal airway patency. Previous recordings of multiunit electromyogram (EMG) suggest it is activated during inspiration in humans with some tonic activity in expiration. We recorded from populations of single motor units in GG in seven subjects during quiet breathing when awake. Ultrasonography assisted electrode placement. The activity of single units was separated into six classes based on a step-wise analysis of the discharge pattern. Phasic and tonic activities were analyzed statistically with the coefficient of determination ( r 2 ) between discharge frequency and lung volume. Of the 110 motor units, 29% discharged tonically without phasic respiratory modulation (firing rate 19 Hz). Further, 16% of units increased their discharge during expiration (expiratory phasic and expiratory tonic units). Only half the units increased their discharge during inspiration (inspiratory phasic and inspiratory tonic units). Units firing tonically with an inspiratory increase had significantly higher discharge rates than those units that only fired phasically (peak rates 25 vs. 16 Hz, respectively). Simultaneous recordings of two or three motor units showed neighboring units with differing respiratory and tonic drives. Our results provide a classification and the first quantitative measures of human GG motor-unit behavior and suggest this activity results from a complex interaction of inspiratory, expiratory, and tonic drives at the hypoglossal motor nucleus. The presence of different drives to GG implies that complex premotor networks can differentially engage human hypoglossal motoneurons during respiration. This is unlike the ordered recruitment of motor units in limb and axial muscles. Address for reprint requests and other correspondence: S. Gandevia, Prince of Wales Medical Research Institute, Barker St, Randwick, Sydney, NSW, 2031, Australia (E-mail: s.gandevia{at}unsw.edu.au )