The tomato [Lycopersicon esculentum] photomorphogenetic mutant, aurea, is deficient in phytochromobilin synthase for phytochrome chromophore biosynthesis

The aurea mutants of tomato have been widely used as phytochrome-deficient mutants for photomorphogenetic and photobiological studies. By expressed sequence tag (EST)-based screening of sequence databases, we found a tomato gene that encodes a protein homologous to Arabidopsis HY2 for phytochromobil...

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Bibliographic Details
Published in:Plant and cell physiology 2005-04, Vol.46 (4), p.661-665
Main Authors: Muramoto, T.(Kyoto Univ. (Japan)), Kami, C, Kataoka, H, Iwata, N, Linley, P.J, Mukougawa, K, Yokota, A, Kohchi, T
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Arabidopsis
Aurea
biliverdin
biosynthesis
chromophore
complementary DNA
Cph1
cyanobacterial phytochrome 1
enzyme activity
ESCHERICHIA COLI
EST
Evolution, Molecular
EXPRESION GENICA
expressed sequence tag
expressed sequence tags
EXPRESSION DES GENES
FITOCROMA
gene deletion
GENE EXPRESSION
genetic recombination
glutathione S-transferase
GST
LYCOPERSICON ESCULENTUM
Lycopersicon esculentum - enzymology
Lycopersicon esculentum - genetics
Molecular Sequence Data
morphogenesis
MUTANT
MUTANTES
MUTANTS
Mutation - physiology
nucleotide sequences
nucleotide substitution
oxidoreductases
Oxidoreductases - deficiency
Oxidoreductases - genetics
Oxidoreductases - metabolism
PCB
PcyA
Photomorphogenesis
photoreceptors
phycocyanobilin
phycocyanobilin:ferredoxin oxidoreductase
Phylogeny
PHYTOCHROME
Phytochrome - genetics
Phytochrome - metabolism
Phytochromobilin
phytochromobilin synthase
plant biochemistry
plant genetics
plant proteins
PΦB
recombinant fusion proteins
Solanum lycopersicum var. lycopersicum
tomatoes
vegetable crops
YELLOW-GREEN-2
YG-2
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Summary:The aurea mutants of tomato have been widely used as phytochrome-deficient mutants for photomorphogenetic and photobiological studies. By expressed sequence tag (EST)-based screening of sequence databases, we found a tomato gene that encodes a protein homologous to Arabidopsis HY2 for phytochromobilin synthase catalyzing the last step of phytochrome chromophore biosynthesis. The tomato protein expressed in Escherichia coli showed phytochromobilin synthase activity. The corresponding loci in all aurea mutants tested have nucleotide substitutions, deletions or DNA rearrangements. These results indicate that aurea is a mutant of phytochromobilin synthase in tomato. We also discuss a phylogenetic analysis of phytochromobilin synthases in the bilin reductase family.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pci062