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Immunogenicity and effect of a virosomal influenza vaccine on viral replication and T-cell activation in HIV-infected children receiving highly active antiretroviral therapy
In order to evaluate the immunogenicity and the effect of a virosomal influenza vaccine on viral replication and T‐cell activation in HIV‐infected children receiving highly active antiretroviral therapy (HAART), 29 children infected with HIV‐1 vertically (19 primed with a previous influenza vaccinat...
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Published in: | Journal of medical virology 2006-04, Vol.78 (4), p.440-445 |
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creator | Tanzi, Elisabetta Esposito, Susanna Bojanin, Jelena Amendola, Antonella Trabattoni, Daria Pariani, Elena Pinzani, Raffaella Zanetti, Alessandro Principi, Nicola |
description | In order to evaluate the immunogenicity and the effect of a virosomal influenza vaccine on viral replication and T‐cell activation in HIV‐infected children receiving highly active antiretroviral therapy (HAART), 29 children infected with HIV‐1 vertically (19 primed with a previous influenza vaccination and 10 who were not been immunized against influenza) were immunized with an intramuscular virosome‐adjuvanted influenza vaccine. According to the European Agency for Evaluation of Medical Products (EMEA) criteria, the immunogenicity of the vaccine was adequate against all three influenza strains (A H1N1, A H3N2, and B) in the primed children, and against A H1N1 and A H3N2 in the unprimed children. After in vitro stimulation with vaccine antigens, the IFN‐γ levels in the peripheral blood mononuclear cells cultures increased significantly from a baseline level of 103.0 ± 229.8 pg/ml to a 30‐day level of 390.7 ± 606.3 pg/ml (P |
doi_str_mv | 10.1002/jmv.20559 |
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According to the European Agency for Evaluation of Medical Products (EMEA) criteria, the immunogenicity of the vaccine was adequate against all three influenza strains (A H1N1, A H3N2, and B) in the primed children, and against A H1N1 and A H3N2 in the unprimed children. After in vitro stimulation with vaccine antigens, the IFN‐γ levels in the peripheral blood mononuclear cells cultures increased significantly from a baseline level of 103.0 ± 229.8 pg/ml to a 30‐day level of 390.7 ± 606.3 pg/ml (P < 0.05), with concentrations significantly higher (P < 0.05) in the primed children than in the unprimed children. No increase in plasma HIV‐1 RNA or HIV‐1 proviral DNA was observed in either subgroup, and the immunophenotype analyses demonstrated that the CD4+ cell counts and percentages, the CD4/CD8 ratio and activated lymphocytes remained stable in either group from baseline to 1 month after each vaccine dose. This study showed that the virosomal influenza vaccine does seem to be immunogenic in the majority of HIV‐infected children receiving HAART and does not induce viral replication or T‐cell activation. Given the possible influenza‐related complications in children infected with HIV, these results support the use of this influenza vaccine in such patients. J. Med. Virol. 78:440–445, 2006. © 2006 Wiley‐Liss, Inc.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.20559</identifier><identifier>PMID: 16482542</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adolescent ; Antibodies, Viral - blood ; Antiretroviral Therapy, Highly Active ; Biological and medical sciences ; Child ; children ; Female ; Fundamental and applied biological sciences. Psychology ; HIV ; HIV Infections - drug therapy ; HIV Infections - immunology ; HIV-1 - drug effects ; HIV-1 - physiology ; Human immunodeficiency virus 1 ; Human viral diseases ; Humans ; immunogenicity ; Infectious diseases ; Influenza A Virus, H1N1 Subtype - immunology ; Influenza A Virus, H3N2 Subtype - immunology ; Influenza B virus - immunology ; influenza vaccine ; Influenza Vaccines - administration & dosage ; Influenza Vaccines - immunology ; Influenza, Human - immunology ; Influenza, Human - prevention & control ; Interferon-gamma - biosynthesis ; Lymphocyte Activation ; Male ; Medical sciences ; Microbiology ; Miscellaneous ; T-cell activation ; T-Lymphocytes - immunology ; Treatment Outcome ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Viral Load ; viral replication ; Virology ; Virosomes ; Virus Replication</subject><ispartof>Journal of medical virology, 2006-04, Vol.78 (4), p.440-445</ispartof><rights>Copyright © 2006 Wiley‐Liss, Inc.</rights><rights>2006 INIST-CNRS</rights><rights>Copyright 2006 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4229-e9f7dbc439a7a8e79e7b5fbf44db94520796f75f2eba785a2ad1198f30b3854e3</citedby><cites>FETCH-LOGICAL-c4229-e9f7dbc439a7a8e79e7b5fbf44db94520796f75f2eba785a2ad1198f30b3854e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17585781$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16482542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tanzi, Elisabetta</creatorcontrib><creatorcontrib>Esposito, Susanna</creatorcontrib><creatorcontrib>Bojanin, Jelena</creatorcontrib><creatorcontrib>Amendola, Antonella</creatorcontrib><creatorcontrib>Trabattoni, Daria</creatorcontrib><creatorcontrib>Pariani, Elena</creatorcontrib><creatorcontrib>Pinzani, Raffaella</creatorcontrib><creatorcontrib>Zanetti, Alessandro</creatorcontrib><creatorcontrib>Principi, Nicola</creatorcontrib><title>Immunogenicity and effect of a virosomal influenza vaccine on viral replication and T-cell activation in HIV-infected children receiving highly active antiretroviral therapy</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>In order to evaluate the immunogenicity and the effect of a virosomal influenza vaccine on viral replication and T‐cell activation in HIV‐infected children receiving highly active antiretroviral therapy (HAART), 29 children infected with HIV‐1 vertically (19 primed with a previous influenza vaccination and 10 who were not been immunized against influenza) were immunized with an intramuscular virosome‐adjuvanted influenza vaccine. According to the European Agency for Evaluation of Medical Products (EMEA) criteria, the immunogenicity of the vaccine was adequate against all three influenza strains (A H1N1, A H3N2, and B) in the primed children, and against A H1N1 and A H3N2 in the unprimed children. After in vitro stimulation with vaccine antigens, the IFN‐γ levels in the peripheral blood mononuclear cells cultures increased significantly from a baseline level of 103.0 ± 229.8 pg/ml to a 30‐day level of 390.7 ± 606.3 pg/ml (P < 0.05), with concentrations significantly higher (P < 0.05) in the primed children than in the unprimed children. No increase in plasma HIV‐1 RNA or HIV‐1 proviral DNA was observed in either subgroup, and the immunophenotype analyses demonstrated that the CD4+ cell counts and percentages, the CD4/CD8 ratio and activated lymphocytes remained stable in either group from baseline to 1 month after each vaccine dose. This study showed that the virosomal influenza vaccine does seem to be immunogenic in the majority of HIV‐infected children receiving HAART and does not induce viral replication or T‐cell activation. 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Psychology</subject><subject>HIV</subject><subject>HIV Infections - drug therapy</subject><subject>HIV Infections - immunology</subject><subject>HIV-1 - drug effects</subject><subject>HIV-1 - physiology</subject><subject>Human immunodeficiency virus 1</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>immunogenicity</subject><subject>Infectious diseases</subject><subject>Influenza A Virus, H1N1 Subtype - immunology</subject><subject>Influenza A Virus, H3N2 Subtype - immunology</subject><subject>Influenza B virus - immunology</subject><subject>influenza vaccine</subject><subject>Influenza Vaccines - administration & dosage</subject><subject>Influenza Vaccines - immunology</subject><subject>Influenza, Human - immunology</subject><subject>Influenza, Human - prevention & control</subject><subject>Interferon-gamma - biosynthesis</subject><subject>Lymphocyte Activation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>T-cell activation</subject><subject>T-Lymphocytes - immunology</subject><subject>Treatment Outcome</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Viral Load</subject><subject>viral replication</subject><subject>Virology</subject><subject>Virosomes</subject><subject>Virus Replication</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkcFu1DAURSNERYfCgh9A3oDEIq3t2HG8hBG0U01hUwZ2luM8z7gkzmBnBsI_8Y94yEBXiJWl-869T883y54RfE4wphd33f6cYs7lg2xGsCxziQV5mM0wYWVeloSfZo9jvMMYV5LSR9kpKVlFOaOz7Oei63a-X4N3xg0j0r5BYC2YAfUWabR3oY99p1vkvG134H8kTRvjPKDeH8ZpFGDbOqMHl5RDwG1uoG2RNoPbT6rz6GqxylNGSoYGmY1rmwA-WQ24vfNrtHHrTTtOJkgxgwswhH7aMGwg6O34JDuxuo3w9PieZR_fvb2dX-XLD5eL-etlbhilMgdpRVMbVkgtdAVCgqi5rS1jTS0Zp1jI0gpuKdRaVFxT3RAiK1vguqg4g-IseznlbkP_dQdxUJ2Lh5u0h34XVSmEZKXk_wVTK7SiDCfw1QSa9J8xgFXb4DodRkWwOpSoUonqd4mJfX4M3dUdNPfksbUEvDgCOhrd2qC9cfGeE7zioiKJu5i4b66F8d8b1fXN6s_qfHK4OMD3vw4dvqSbC8HVp_eXqpzffCbLNyu1Kn4BY_fG5A</recordid><startdate>200604</startdate><enddate>200604</enddate><creator>Tanzi, Elisabetta</creator><creator>Esposito, Susanna</creator><creator>Bojanin, Jelena</creator><creator>Amendola, Antonella</creator><creator>Trabattoni, Daria</creator><creator>Pariani, Elena</creator><creator>Pinzani, Raffaella</creator><creator>Zanetti, Alessandro</creator><creator>Principi, Nicola</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200604</creationdate><title>Immunogenicity and effect of a virosomal influenza vaccine on viral replication and T-cell activation in HIV-infected children receiving highly active antiretroviral therapy</title><author>Tanzi, Elisabetta ; Esposito, Susanna ; Bojanin, Jelena ; Amendola, Antonella ; Trabattoni, Daria ; Pariani, Elena ; Pinzani, Raffaella ; Zanetti, Alessandro ; Principi, Nicola</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4229-e9f7dbc439a7a8e79e7b5fbf44db94520796f75f2eba785a2ad1198f30b3854e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Adolescent</topic><topic>Antibodies, Viral - blood</topic><topic>Antiretroviral Therapy, Highly Active</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>children</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV</topic><topic>HIV Infections - drug therapy</topic><topic>HIV Infections - immunology</topic><topic>HIV-1 - drug effects</topic><topic>HIV-1 - physiology</topic><topic>Human immunodeficiency virus 1</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>immunogenicity</topic><topic>Infectious diseases</topic><topic>Influenza A Virus, H1N1 Subtype - immunology</topic><topic>Influenza A Virus, H3N2 Subtype - immunology</topic><topic>Influenza B virus - immunology</topic><topic>influenza vaccine</topic><topic>Influenza Vaccines - administration & dosage</topic><topic>Influenza Vaccines - immunology</topic><topic>Influenza, Human - immunology</topic><topic>Influenza, Human - prevention & control</topic><topic>Interferon-gamma - biosynthesis</topic><topic>Lymphocyte Activation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>T-cell activation</topic><topic>T-Lymphocytes - immunology</topic><topic>Treatment Outcome</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Viral Load</topic><topic>viral replication</topic><topic>Virology</topic><topic>Virosomes</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanzi, Elisabetta</creatorcontrib><creatorcontrib>Esposito, Susanna</creatorcontrib><creatorcontrib>Bojanin, Jelena</creatorcontrib><creatorcontrib>Amendola, Antonella</creatorcontrib><creatorcontrib>Trabattoni, Daria</creatorcontrib><creatorcontrib>Pariani, Elena</creatorcontrib><creatorcontrib>Pinzani, Raffaella</creatorcontrib><creatorcontrib>Zanetti, Alessandro</creatorcontrib><creatorcontrib>Principi, Nicola</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanzi, Elisabetta</au><au>Esposito, Susanna</au><au>Bojanin, Jelena</au><au>Amendola, Antonella</au><au>Trabattoni, Daria</au><au>Pariani, Elena</au><au>Pinzani, Raffaella</au><au>Zanetti, Alessandro</au><au>Principi, Nicola</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunogenicity and effect of a virosomal influenza vaccine on viral replication and T-cell activation in HIV-infected children receiving highly active antiretroviral therapy</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2006-04</date><risdate>2006</risdate><volume>78</volume><issue>4</issue><spage>440</spage><epage>445</epage><pages>440-445</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>In order to evaluate the immunogenicity and the effect of a virosomal influenza vaccine on viral replication and T‐cell activation in HIV‐infected children receiving highly active antiretroviral therapy (HAART), 29 children infected with HIV‐1 vertically (19 primed with a previous influenza vaccination and 10 who were not been immunized against influenza) were immunized with an intramuscular virosome‐adjuvanted influenza vaccine. According to the European Agency for Evaluation of Medical Products (EMEA) criteria, the immunogenicity of the vaccine was adequate against all three influenza strains (A H1N1, A H3N2, and B) in the primed children, and against A H1N1 and A H3N2 in the unprimed children. After in vitro stimulation with vaccine antigens, the IFN‐γ levels in the peripheral blood mononuclear cells cultures increased significantly from a baseline level of 103.0 ± 229.8 pg/ml to a 30‐day level of 390.7 ± 606.3 pg/ml (P < 0.05), with concentrations significantly higher (P < 0.05) in the primed children than in the unprimed children. No increase in plasma HIV‐1 RNA or HIV‐1 proviral DNA was observed in either subgroup, and the immunophenotype analyses demonstrated that the CD4+ cell counts and percentages, the CD4/CD8 ratio and activated lymphocytes remained stable in either group from baseline to 1 month after each vaccine dose. This study showed that the virosomal influenza vaccine does seem to be immunogenic in the majority of HIV‐infected children receiving HAART and does not induce viral replication or T‐cell activation. Given the possible influenza‐related complications in children infected with HIV, these results support the use of this influenza vaccine in such patients. J. Med. Virol. 78:440–445, 2006. © 2006 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>16482542</pmid><doi>10.1002/jmv.20559</doi><tpages>6</tpages></addata></record> |
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subjects | Adolescent Antibodies, Viral - blood Antiretroviral Therapy, Highly Active Biological and medical sciences Child children Female Fundamental and applied biological sciences. Psychology HIV HIV Infections - drug therapy HIV Infections - immunology HIV-1 - drug effects HIV-1 - physiology Human immunodeficiency virus 1 Human viral diseases Humans immunogenicity Infectious diseases Influenza A Virus, H1N1 Subtype - immunology Influenza A Virus, H3N2 Subtype - immunology Influenza B virus - immunology influenza vaccine Influenza Vaccines - administration & dosage Influenza Vaccines - immunology Influenza, Human - immunology Influenza, Human - prevention & control Interferon-gamma - biosynthesis Lymphocyte Activation Male Medical sciences Microbiology Miscellaneous T-cell activation T-Lymphocytes - immunology Treatment Outcome Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Viral Load viral replication Virology Virosomes Virus Replication |
title | Immunogenicity and effect of a virosomal influenza vaccine on viral replication and T-cell activation in HIV-infected children receiving highly active antiretroviral therapy |
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