Reevaluation of the resting time period when preparing whole blood-derived platelet concentrates with the platelet-rich plasma method

• Published in: Transfusion (Philadelphia, Pa.) Pa.), 2006-04, Vol.46 (4), p.572-577
• Main Authors: Moroff, Gary, Kline, Linda, Dabay, Michelle, Hunter, Suzanne, Johnson, Adrienne, McNeil, Deanna, Nixon, Jan, Sawyer, Sherrie, Taylor, Harry, Whitley, Pam, Wahab, Fawzi, Murphy, Scott
• Format: Article
• Language: English
• Subjects: Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Anticoagulants; Biological and medical sciences; Blood coagulation. Blood cells; Blood Platelets - cytology; Blood Preservation - methods; Blood. Blood and plasma substitutes. Blood products. Blood cells. Blood typing. Plasmapheresis. Apheresis; Carbon Dioxide - blood; Cell Survival; Emergency and intensive cardiocirculatory care. Cardiogenic shock. Coronary intensive care; Fundamental and applied biological sciences. Psychology; Humans; In Vitro Techniques; Intensive care medicine; Medical sciences; Molecular and cellular biology; Oxygen - blood; Platelet; Platelet Count; Platelet Transfusion - methods; Time Factors; Transfusions. Complications. Transfusion reactions. Cell and gene therapy
• ISSN: 0041-1132; 1537-2995
• DOI: 10.1111/j.1537-2995.2006.00756.x

BACKGROUND:  The resting of platelet (PLT) pellets during the preparation of whole blood–derived PLT concentrates (PCs) is considered an essential step. A reevaluation of the rest period was conducted because preparation and storage conditions have been modified during the past 20 years. STUDY DESIGN AND METHODS:  A two‐site in vitro study (Study 1) was conducted with rest times of 0 to 5 minutes, 1 hour, and 4 hours (n = 31‐33 per rest period). Leukoreduced PCs were stored for 5 days. A second study (Study 2) measuring in vivo viability was conducted at a third site (14 paired studies). PCs were prepared from 2 units of whole blood on the same day to simultaneously compare viability following storage and radioisotopic labeling. RESULTS:  In Study 1, comparable in vitro parameters and swirling were observed with the three rest periods. The mean (±1 SD) values after storage for the extent of shape change and hypotonic stress parameters were 0 to 5 minutes, 16.7 ± 7.2 and 66.0 ± 15.7%; 1 hour, 19.1 ± 6.9 and 69.3 ± 17.9%; and 4 hours, 17.6 ± 5.5 and 64.1 ± 11.3%. In Study 2, the in vivo recovery was 49.9 ± 15.3 and 50.9 ± 20.2% with 0‐ to 5‐minute and 1‐hour rest periods, respectively. The corresponding survival time was 111.2 ± 50.7 and 114.9 ± 43.8 hours. CONCLUSION:  These studies indicate comparable in vitro and in vivo viability properties with 0‐ to 5‐minute and 1‐hour rest periods and at 4 hours (in vitro only).