The effects of analytical factors on second trimester risk estimations

Objective: Triple test with measured maternal serum α-fetoprotein, human chorionic gonadotropin, and unconjugated estriol combination as a routine procedure for fetal Down's syndrome, trisomy 18 and neural tube defect screening has some intrinsic problems, such as precision. The aim of this stu...

Full description

Saved in:
Bibliographic Details
Published in:International journal of gynecology and obstetrics 2006-04, Vol.93 (1), p.28-32
Main Authors: Serdar, M.A., Tütüncü, L., Olgun, A., Haşimi, A., Ozgurtaş, T., Erbil, M.K.
Format: Article
Language:English
Subjects:
Adult
alpha-Fetoproteins - analysis
Biological and medical sciences
Biomarkers - blood
Chorionic Gonadotropin - analysis
Chromosome aberrations
Chromosomes, Human, 16-18
Down Syndrome - diagnosis
Down's syndrome
Estriol - analysis
Evaluation Studies as Topic
Female
Gynecology. Andrology. Obstetrics
Humans
Mass Screening
Medical genetics
Medical sciences
Neural Tube Defects - diagnosis
Predictive Value of Tests
Pregnancy
Pregnancy Outcome
Pregnancy Trimester, Second
Risk estimation
Triple test
Trisomy - diagnosis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective: Triple test with measured maternal serum α-fetoprotein, human chorionic gonadotropin, and unconjugated estriol combination as a routine procedure for fetal Down's syndrome, trisomy 18 and neural tube defect screening has some intrinsic problems, such as precision. The aim of this study was to evaluate the effect of analytical variation of triple test on prenatal risk estimation. Method: Five different serum pools were prepared and triple test was performed seven times for within run and five times for between run precision determination. Result: Within run and between run, precision values of risk estimations by measuring the same sample for Triple test were calculated to be 7.9–21.4% and 14.1–31.0% for trisomy 21, 13.2–23.7% and 14.2–15.1% for trisomy 18, 47.2 and 42.0 % for neural tube defect, respectively. Conclusion: These results demonstrated that analytical variations have great impact on second trimester risk estimation procedures; therefore, triple test analyses should be carried out in laboratories using strict internal and external quality control programs. Moreover, triple test results should always be interpreted by considering analytical and biological variations.
ISSN:0020-7292
1879-3479
DOI:10.1016/j.ijgo.2005.12.026