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Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification

1 Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany 2 Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Spain 3 Centro de Investigación Agropecuaria, El Deheson del Encinar, Oropesa, Spain Corresp...

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Published in:Journal of general virology 2006-05, Vol.87 (5), p.1189-1195
Main Authors: Johne, Reimar, Fernandez-de-Luco, Daniel, Hofle, Ursula, Muller, Hermann
Format: Article
Language:English
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Summary:1 Institute for Virology, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 29, D-04103 Leipzig, Germany 2 Department of Animal Pathology, Veterinary Faculty, University of Zaragoza, Spain 3 Centro de Investigación Agropecuaria, El Deheson del Encinar, Oropesa, Spain Correspondence Reimar Johne johne{at}vetmed.uni-leipzig.de The genus Circovirus comprises small non-enveloped viruses with a circular single-stranded DNA genome. By using PCR with degenerate primers, a novel circovirus (starling circovirus, StCV) was detected in spleen samples of wild starlings ( Sturnus vulgaris and Sturnus unicolor ) found dead during an epidemic outbreak of septicaemic salmonellosis in northeastern Spain. Using a specific PCR, StCV was also detected in apparently healthy birds from the same population. The genome was amplified using multiply primed rolling-circle amplification and cloned. Open reading frames (ORFs) with similarities to the replication-associated protein and the capsid protein of circoviruses as well as an additional ORF encoding a protein of 106 aa were evident from the sequence. Phylogenetic analysis of circovirus genomes revealed the highest degree of similarity (67·1 %) between StCV and canary circovirus. A similar analysis of the evolutionarily conserved cytochrome b gene of the circovirus host species revealed a strict co-evolution of circoviruses with their hosts; however, the circoviruses showed about a threefold higher genetic divergence than their hosts. The GenBank/EMBL/DDBJ accession number of the sequence reported in this paper is DQ172906 [GenBank] .
ISSN:0022-1317
1465-2099
DOI:10.1099/vir.0.81561-0