Detection of recurrent copy number loss at Yp11.2 involving TSPY gene cluster in prostate cancer using array-based comparative genomic hybridization

Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 2006-04, Vol.66 (8), p.4055-4064
Main Authors: VIJAYAKUMAR, Sapna, HALL, Devon C, REVELES, Xavier T, TROYER, Dean A, THOMPSON, Ian M, GARCIA, Dawn, RUIHUA XIANG, LEACH, Robin J, JOHNSON-PAIS, Teresa L, NAYLOR, Susan L
Format: Article
Language:English
Subjects:
Aged
Antineoplastic agents
Biological and medical sciences
Cell Cycle Proteins - genetics
Cell Line, Tumor
Chromosomes, Human, Y - genetics
Gene Dosage
Gynecology. Andrology. Obstetrics
Humans
Male
Male genital diseases
Medical sciences
Middle Aged
Multigene Family
Neoplasm Staging
Nephrology. Urinary tract diseases
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Array Sequence Analysis - standards
Pharmacology. Drug treatments
Prostatic Neoplasms - genetics
Prostatic Neoplasms - pathology
Reverse Transcriptase Polymerase Chain Reaction
Tumors
Tumors of the urinary system
Urinary tract. Prostate gland
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Summary:Prostate cancer is the second leading cause of cancer deaths among American men. The loss of Y chromosome has been frequently observed in primary prostate cancer as well as other types of cancer. Earlier, we showed that introduction of the human Y chromosome suppresses the in vivo tumorigenicity of the prostate cancer cell line PC-3. To further characterize the Y chromosome, we have developed a high-density bacterial artificial chromosome (BAC) microarray containing 178 BAC clones from the human Y chromosome. BAC microarray was used for array comparative genomic hybridization on prostate cancer samples and cell lines. The most prominent observation on prostate cancer specimens was a deletion at Yp11.2 containing the TSPY tandem gene array. Out of 36 primary prostate tumors analyzed, 16 (44.4%) samples exhibited loss of TSPY gene copies. Notably, we observed association between the number of TSPY copies in the blood and the incidence of prostate cancer. Moreover, PC-3 hybrids with an intact Yp11.2 did not grow tumors in nude mice, whereas PC-3 hybrids with a deletion at Yp11.2 grew tumors in nude mice.
ISSN:0008-5472
1538-7445
DOI:10.1158/0008-5472.CAN-05-3822