ISSR, ERIC and RAPD techniques to detect genetic diversity in the aphid pathogen Pandora neoaphidis

The entomopathogenic fungus Pandora neoaphidis is an important natural enemy of aphids. ISSR, ERIC (Enterobacterial Repetitive Intergenic Consensus) and RAPD PCR-based DNA fingerprint analyses were undertaken to study intra-specific variation amongst 30 isolates of P. neoaphidis worldwide, together...

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Bibliographic Details
Published in:Mycological research 2005-03, Vol.109 (3), p.285-293
Main Authors: TYMON, Anna M., PELL, Judith K.
Format: Article
Language:English
Subjects:
Animals
Aphidoidea
Aphids - microbiology
biological control agents
DNA fingerprinting
DNA, Fungal - analysis
DNA, Fungal - chemistry
entomopathogenic fungi
Entomophthorales
Entomophthorales - genetics
Entomophthorales - isolation & purification
Erynia neoaphidis
Genetic Variation
intraspecific variation
Microlophium
Microlophium carnosum
molecular systematics
Pandora neoaphidis
phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
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Summary:The entomopathogenic fungus Pandora neoaphidis is an important natural enemy of aphids. ISSR, ERIC (Enterobacterial Repetitive Intergenic Consensus) and RAPD PCR-based DNA fingerprint analyses were undertaken to study intra-specific variation amongst 30 isolates of P. neoaphidis worldwide, together with six closely related species of Entomophthorales. All methods yielded scorable binary characters, and distance matrices were constructed from both individual and combined data sets. Neighbour-joining was used to construct consensus phylogenetic trees which showed that although P. neoaphidis isolates were highly polymorphic they separated into a monophyletic group compared with the other Entomophthorales tested. Three distinct subclades were found, with UK isolates occupying two of these. No specific correlation with aphid host species was established for any of the isolates apart from those in one cluster which contained isolates obtained from nettle aphid, Microlophium carnosum. ERIC, ISSR and RAPD analysis allowed the rapid genetic characterisation and differentiation of isolates with the generation of potential isolate- and cluster specific- diagnostic DNA markers.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756204001807