Ovariectomy enhances acetylcholinesterase activity but does not alter ganglioside content in cerebral cortex of female adult rats

In the present work we investigated the effect of ovariectomy on acetylcholinesterase (AChE) activity and ganglioside content in cerebral cortex of female rats. We also studied the activity of butyrylcholinesterase (BuChE) in serum of these animals. Adult Wistar rats were divided into three groups:...

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Published in:Metabolic brain disease 2005-03, Vol.20 (1), p.35-44
Main Authors: MONTEIRO, Siomara C, STEFANELLO, Francieli M, VIANNA, Luciene P, MATTE, Cristiane, BARP, Jaqueline, BELLO-KLEIN, Adriane, TRINDADE, Vera M. T, WYSE, Angela T. S
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
Acetylcholinesterase - metabolism
Animals
Biological and medical sciences
Butyrylcholinesterase - blood
Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges
Cerebral Cortex - enzymology
Cerebral Cortex - metabolism
Chromatography, Thin Layer
Disease Models, Animal
Down-Regulation - physiology
Female
Fundamental and applied biological sciences. Psychology
Gangliosides - metabolism
Medical sciences
Neurology
Neuropharmacology
Neuroprotective agent
Ovariectomy
Pharmacology. Drug treatments
Postmenopause - metabolism
Rats
Rats, Wistar
Up-Regulation - physiology
Vertebrates: nervous system and sense organs
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Summary:In the present work we investigated the effect of ovariectomy on acetylcholinesterase (AChE) activity and ganglioside content in cerebral cortex of female rats. We also studied the activity of butyrylcholinesterase (BuChE) in serum of these animals. Adult Wistar rats were divided into three groups: (1) naive females (control), (2) sham-operated females and (3) castrated females (ovariectomy). Thirty days after ovariectomy, rats were sacrificed by decapitation without anaesthesia. Blood was collected and the serum used for BuChE determination. Cerebral cortex was homogenized to determine AChE activity and extracted with chlorophorm:methanol for ganglioside evaluation. Results showed that rats subjected to ovariectomy presented a significant increase of AChE activity, but did not change the content and the profile of gangliosides in cerebral cortex when compared to sham or naive rats. BuChE activity was decreased in serum of rats ovariectomized. Our findings suggest that the alteration in the activity of brain AChE, as well as serum BuChE activity caused by ovariectomy may contribute to the impaired cognition and/or other neurological dysfunction found in post-menopausal women.
ISSN:0885-7490
1573-7365
DOI:10.1007/s11011-005-2474-1