Highly sensitive analysis of the anti-tumor agent 1-[4-(furo[2,3-b]-quinolin-4-ylamino)phenyl]ethanone in rat plasma by high-performance liquid chromatography using electrochemical detection

• Published in: Journal of pharmaceutical and biomedical analysis 2005-07, Vol.38 (3), p.551-555
• Main Authors: Huang, Yaw-Bin, Wu, Pao-Chu, Hsu, Ming-Wei, Chen, Yeh-Long, Tzeng, Cherng-Chyi, Tsai, Yi-Hung
• Format: Article
• Language: English
• Subjects: 1-[4-(furo[2,3-b]-quinolin-4-ylamino)phenyl]ethanone; Amsacrine; Amsacrine - blood; Amsacrine - chemistry; Amsacrine - pharmacokinetics; Analysis; Analytical, structural and metabolic biochemistry; Animals; Anti-tumor; Antineoplastic Agents - blood; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacokinetics; Area Under Curve; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Drug Screening Assays, Antitumor - methods; Electrochemistry - methods; Fundamental and applied biological sciences. Psychology; General pharmacology; Half-Life; Medical sciences; Molecular Structure; Pharmacokinetics; Pharmacology. Drug treatments; Quinolines - blood; Quinolines - chemistry; Quinolines - pharmacokinetics; Rats; Rats, Wistar; Reproducibility of Results; Technology, Pharmaceutical - methods
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2005.01.018

A sensitive high-performance liquid chromatography method with electrochemical detection was developed for the purpose of determining the concentration of the new anti-tumor agent 1-[4-(furo[2,3-b]-quinolin-4-ylamino)phenyl]ethanone (FQPE) in rats. The plasma samples were spiked with the internal standard diclofenac and extracted using dichloromethane. A C 18 250 mm × 4 mm column was used for the separation of analyte with a mobile phase consisting of 50% acetonitrile and 50% pH 3.0 of sodium 1-pentansulfonate solution at a flow rate of 1.0 mL/min. FQPE was detected by electrochemical detector at 1.0 V and 20 nA. Intra-day and inter-day precision and accuracy were acceptable down to the limit of quantization of 1 ng/mL. The lower limit of detection (LOD) was 0.5 ng/mL. The pharmacokinetic parameters of FQPE in rats after intravenous administration of 2.1 and 4.2 mg/kg were determined. The apparent volume of distribution, half-life of elimination, and clearance showed no significant difference between the two dosages. The area under the plasma concentration time curve increased proportionally with dose. The half-life of FQPE was prolonged about 2.4-fold, compared with amsacrine.