Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells

Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the e...

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Published in:Circulation (New York, N.Y.) N.Y.), 2005-05, Vol.111 (21), p.2792-2797
Main Authors: RIEDER, Erwin, SEEBACHER, Gernot, KASIMIR, Marie-Theres, EICHMAIR, Eva, WINTER, Birgitta, DEKAN, Barbara, WOLNER, Ernst, SIMON, Paul, WEIGEL, Guenter
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blood and lymphatic vessels
Blood vessels and receptors
Cardiology. Vascular system
Cardiovascular system
Cell Line
Cell Movement
Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Heart Valves - chemistry
Heart Valves - cytology
Heart Valves - immunology
Humans
Medical sciences
Monocytes - physiology
Pharmacology. Drug treatments
Proteins - immunology
Proteins - isolation & purification
Species Specificity
Swine
Tissue Engineering - methods
Vasodilator agents. Cerebral vasodilators
Vertebrates: cardiovascular system
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Summary:Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the early immunologic reaction. Porcine and human pulmonary valve conduits were decellularized, and migration of U-937 monocytic cells toward extracted heart valve proteins was examined in a transmigration chamber in vitro. Homogenized tissue specimens were size fractionated by SDS-PAGE. The decellularization procedure effectively reduced the migration of human monocytes toward all heart valve tissue. However, only the antigen reduction of human pulmonary valves abolished the monocytic response (wall, 0.88+/-0.19% versus 30.20+/-3.93% migrated cells [mean+/-SEM]; cusps, 0.10+/-0.06% versus 10.24+/-1.83%) and was significantly lower (P
ISSN:0009-7322
1524-4539
DOI:10.1161/CIRCULATIONAHA.104.473629