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Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells
Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the e...
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| Published in: | Circulation (New York, N.Y.) N.Y.), 2005-05, Vol.111 (21), p.2792-2797 |
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| Language: | English |
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| container_end_page | 2797 |
| container_issue | 21 |
| container_start_page | 2792 |
| container_title | Circulation (New York, N.Y.) |
| container_volume | 111 |
| creator | RIEDER, Erwin SEEBACHER, Gernot KASIMIR, Marie-Theres EICHMAIR, Eva WINTER, Birgitta DEKAN, Barbara WOLNER, Ernst SIMON, Paul WEIGEL, Guenter |
| description | Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the early immunologic reaction.
Porcine and human pulmonary valve conduits were decellularized, and migration of U-937 monocytic cells toward extracted heart valve proteins was examined in a transmigration chamber in vitro. Homogenized tissue specimens were size fractionated by SDS-PAGE. The decellularization procedure effectively reduced the migration of human monocytes toward all heart valve tissue. However, only the antigen reduction of human pulmonary valves abolished the monocytic response (wall, 0.88+/-0.19% versus 30.20+/-3.93% migrated cells [mean+/-SEM]; cusps, 0.10+/-0.06% versus 10.24+/-1.83%) and was significantly lower (P |
| doi_str_mv | 10.1161/CIRCULATIONAHA.104.473629 |
| format | article |
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Porcine and human pulmonary valve conduits were decellularized, and migration of U-937 monocytic cells toward extracted heart valve proteins was examined in a transmigration chamber in vitro. Homogenized tissue specimens were size fractionated by SDS-PAGE. The decellularization procedure effectively reduced the migration of human monocytes toward all heart valve tissue. However, only the antigen reduction of human pulmonary valves abolished the monocytic response (wall, 0.88+/-0.19% versus 30.20+/-3.93% migrated cells [mean+/-SEM]; cusps, 0.10+/-0.06% versus 10.24+/-1.83%) and was significantly lower (P<0.05) than that of the decellularized porcine equivalent (wall, 5.03+/-0.14% versus 24.31+/-2.38%; cusps, 3.18+/-0.38% versus 10.24+/-1.83%). SDS-PAGE of the pulmonary heart valve tissue revealed that considerable amounts of proteins with different molecular weights that were not detected in the human equivalent remain in the decellularized porcine heart valve.
We describe for the first time that the remaining potential of decellularized pulmonary heart valves to attract monocytic cells depends strongly on whether porcine or human scaffolds were used. These findings will have an important impact on further investigations in the field of heart valve tissue engineering.</description><identifier>ISSN: 0009-7322</identifier><identifier>EISSN: 1524-4539</identifier><identifier>DOI: 10.1161/CIRCULATIONAHA.104.473629</identifier><identifier>PMID: 15911701</identifier><identifier>CODEN: CIRCAZ</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Animals ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood vessels and receptors ; Cardiology. Vascular system ; Cardiovascular system ; Cell Line ; Cell Movement ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Heart Valves - chemistry ; Heart Valves - cytology ; Heart Valves - immunology ; Humans ; Medical sciences ; Monocytes - physiology ; Pharmacology. Drug treatments ; Proteins - immunology ; Proteins - isolation & purification ; Species Specificity ; Swine ; Tissue Engineering - methods ; Vasodilator agents. Cerebral vasodilators ; Vertebrates: cardiovascular system</subject><ispartof>Circulation (New York, N.Y.), 2005-05, Vol.111 (21), p.2792-2797</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c363t-5a2babee7c6e48d96c77f5d56e278eff781fafc03df023349b1b51e9116455253</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16862368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15911701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>RIEDER, Erwin</creatorcontrib><creatorcontrib>SEEBACHER, Gernot</creatorcontrib><creatorcontrib>KASIMIR, Marie-Theres</creatorcontrib><creatorcontrib>EICHMAIR, Eva</creatorcontrib><creatorcontrib>WINTER, Birgitta</creatorcontrib><creatorcontrib>DEKAN, Barbara</creatorcontrib><creatorcontrib>WOLNER, Ernst</creatorcontrib><creatorcontrib>SIMON, Paul</creatorcontrib><creatorcontrib>WEIGEL, Guenter</creatorcontrib><title>Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells</title><title>Circulation (New York, N.Y.)</title><addtitle>Circulation</addtitle><description>Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the early immunologic reaction.
Porcine and human pulmonary valve conduits were decellularized, and migration of U-937 monocytic cells toward extracted heart valve proteins was examined in a transmigration chamber in vitro. Homogenized tissue specimens were size fractionated by SDS-PAGE. The decellularization procedure effectively reduced the migration of human monocytes toward all heart valve tissue. However, only the antigen reduction of human pulmonary valves abolished the monocytic response (wall, 0.88+/-0.19% versus 30.20+/-3.93% migrated cells [mean+/-SEM]; cusps, 0.10+/-0.06% versus 10.24+/-1.83%) and was significantly lower (P<0.05) than that of the decellularized porcine equivalent (wall, 5.03+/-0.14% versus 24.31+/-2.38%; cusps, 3.18+/-0.38% versus 10.24+/-1.83%). SDS-PAGE of the pulmonary heart valve tissue revealed that considerable amounts of proteins with different molecular weights that were not detected in the human equivalent remain in the decellularized porcine heart valve.
We describe for the first time that the remaining potential of decellularized pulmonary heart valves to attract monocytic cells depends strongly on whether porcine or human scaffolds were used. These findings will have an important impact on further investigations in the field of heart valve tissue engineering.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood vessels and receptors</subject><subject>Cardiology. Vascular system</subject><subject>Cardiovascular system</subject><subject>Cell Line</subject><subject>Cell Movement</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart Valves - chemistry</subject><subject>Heart Valves - cytology</subject><subject>Heart Valves - immunology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Monocytes - physiology</subject><subject>Pharmacology. Drug treatments</subject><subject>Proteins - immunology</subject><subject>Proteins - isolation & purification</subject><subject>Species Specificity</subject><subject>Swine</subject><subject>Tissue Engineering - methods</subject><subject>Vasodilator agents. Cerebral vasodilators</subject><subject>Vertebrates: cardiovascular system</subject><issn>0009-7322</issn><issn>1524-4539</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkctqGzEUhkVpaNykr1DURbsbV5eRNOrOuJcYTALBWQ8azVGiMqNxJU3AfZO-bWVsCF11dS585_oj9IGSJaWSfl5v7tcP29Vuc3e7ulktKamXteKS6VdoQQWrq1pw_RotCCG6UpyxS_Q2pZ8llFyJN-iSCk2pInSB_ux8SjNgCI8-AEQfHvHk8BOYmPGzGZ4h4S_4K1gYhnkw0f-GHu-naAuNTejx0zyacCJxssa5aegT7r1zELEfC5pNyMMB-4AjJN_PZigNMoTsi5cnbHKOxmY8TmGyh-wtPg5L1-jCmSHBu7O9Qg_fv-3WN9X27sdmvdpWlkueK2FYZzoAZSXUTa-lVcqJXkhgqgHnVEOdcZbw3hHGea072gkK5X5ZC8EEv0KfTn33cfo1Q8rt6NNxAxNgmlMrVdNowvl_QUZ0U0upC6hPoI1TShFcu49-NPHQUtIeBWz_FbCk6_YkYKl9fx4ydyP0L5VnxQrw8QyY8u7BRROsTy-cbCTjsuF_AdYMqWk</recordid><startdate>20050531</startdate><enddate>20050531</enddate><creator>RIEDER, Erwin</creator><creator>SEEBACHER, Gernot</creator><creator>KASIMIR, Marie-Theres</creator><creator>EICHMAIR, Eva</creator><creator>WINTER, Birgitta</creator><creator>DEKAN, Barbara</creator><creator>WOLNER, Ernst</creator><creator>SIMON, Paul</creator><creator>WEIGEL, Guenter</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050531</creationdate><title>Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells</title><author>RIEDER, Erwin ; SEEBACHER, Gernot ; KASIMIR, Marie-Theres ; EICHMAIR, Eva ; WINTER, Birgitta ; DEKAN, Barbara ; WOLNER, Ernst ; SIMON, Paul ; WEIGEL, Guenter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-5a2babee7c6e48d96c77f5d56e278eff781fafc03df023349b1b51e9116455253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood vessels and receptors</topic><topic>Cardiology. Vascular system</topic><topic>Cardiovascular system</topic><topic>Cell Line</topic><topic>Cell Movement</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart Valves - chemistry</topic><topic>Heart Valves - cytology</topic><topic>Heart Valves - immunology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Monocytes - physiology</topic><topic>Pharmacology. Drug treatments</topic><topic>Proteins - immunology</topic><topic>Proteins - isolation & purification</topic><topic>Species Specificity</topic><topic>Swine</topic><topic>Tissue Engineering - methods</topic><topic>Vasodilator agents. Cerebral vasodilators</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RIEDER, Erwin</creatorcontrib><creatorcontrib>SEEBACHER, Gernot</creatorcontrib><creatorcontrib>KASIMIR, Marie-Theres</creatorcontrib><creatorcontrib>EICHMAIR, Eva</creatorcontrib><creatorcontrib>WINTER, Birgitta</creatorcontrib><creatorcontrib>DEKAN, Barbara</creatorcontrib><creatorcontrib>WOLNER, Ernst</creatorcontrib><creatorcontrib>SIMON, Paul</creatorcontrib><creatorcontrib>WEIGEL, Guenter</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Circulation (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RIEDER, Erwin</au><au>SEEBACHER, Gernot</au><au>KASIMIR, Marie-Theres</au><au>EICHMAIR, Eva</au><au>WINTER, Birgitta</au><au>DEKAN, Barbara</au><au>WOLNER, Ernst</au><au>SIMON, Paul</au><au>WEIGEL, Guenter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells</atitle><jtitle>Circulation (New York, N.Y.)</jtitle><addtitle>Circulation</addtitle><date>2005-05-31</date><risdate>2005</risdate><volume>111</volume><issue>21</issue><spage>2792</spage><epage>2797</epage><pages>2792-2797</pages><issn>0009-7322</issn><eissn>1524-4539</eissn><coden>CIRCAZ</coden><abstract>Tissue-engineered or decellularized heart valves have already been implanted in humans or are currently approaching the clinical setting. The aim of this study was to examine the migratory response of human monocytic cells toward decellularized porcine and human heart valves, a pivotal step in the early immunologic reaction.
Porcine and human pulmonary valve conduits were decellularized, and migration of U-937 monocytic cells toward extracted heart valve proteins was examined in a transmigration chamber in vitro. Homogenized tissue specimens were size fractionated by SDS-PAGE. The decellularization procedure effectively reduced the migration of human monocytes toward all heart valve tissue. However, only the antigen reduction of human pulmonary valves abolished the monocytic response (wall, 0.88+/-0.19% versus 30.20+/-3.93% migrated cells [mean+/-SEM]; cusps, 0.10+/-0.06% versus 10.24+/-1.83%) and was significantly lower (P<0.05) than that of the decellularized porcine equivalent (wall, 5.03+/-0.14% versus 24.31+/-2.38%; cusps, 3.18+/-0.38% versus 10.24+/-1.83%). SDS-PAGE of the pulmonary heart valve tissue revealed that considerable amounts of proteins with different molecular weights that were not detected in the human equivalent remain in the decellularized porcine heart valve.
We describe for the first time that the remaining potential of decellularized pulmonary heart valves to attract monocytic cells depends strongly on whether porcine or human scaffolds were used. These findings will have an important impact on further investigations in the field of heart valve tissue engineering.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>15911701</pmid><doi>10.1161/CIRCULATIONAHA.104.473629</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0009-7322 |
| ispartof | Circulation (New York, N.Y.), 2005-05, Vol.111 (21), p.2792-2797 |
| issn | 0009-7322 1524-4539 |
| language | eng |
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| source | EZB Electronic Journals Library |
| subjects | Animals Biological and medical sciences Blood and lymphatic vessels Blood vessels and receptors Cardiology. Vascular system Cardiovascular system Cell Line Cell Movement Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Heart Valves - chemistry Heart Valves - cytology Heart Valves - immunology Humans Medical sciences Monocytes - physiology Pharmacology. Drug treatments Proteins - immunology Proteins - isolation & purification Species Specificity Swine Tissue Engineering - methods Vasodilator agents. Cerebral vasodilators Vertebrates: cardiovascular system |
| title | Tissue engineering of heart valves : Decellularized porcine and human valve scaffolds differ importantly in residual potential to attract monocytic cells |
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