Task-based imaging of colon cancer in the Apc(Min/+) mouse model

Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The A...

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Bibliographic Details
Published in:Applied optics (2004) 2006-05, Vol.45 (13), p.3049-3062
Main Authors: McNally, James B, Kirkpatrick, Nathaniel D, Hariri, Lida P, Tumlinson, Alexandre R, Besselsen, David G, Gerner, Eugene W, Utzinger, Urs, Barton, Jennifer K
Format: Article
Language:English
Subjects:
Adenomatous Polyposis Coli Protein - deficiency
Animals
Colonic Neoplasms - metabolism
Colonic Neoplasms - pathology
Disease Models, Animal
Equipment Design
Equipment Failure Analysis
Image Interpretation, Computer-Assisted - methods
Mice
Mice, Inbred C57BL
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Systems Integration
Tomography, Optical Coherence - instrumentation
Tomography, Optical Coherence - methods
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Summary:Optical coherence tomography (OCT), laser-induced fluorescence (LIF), and laser-scanning confocal microscopy (LSCM) were used for the task of multimodal study of healthy and adenomatous mouse colon. The results from each modality were compared with histology, which served as the gold standard. The Apc(Min/+) genetic mouse model of colon cancer was compared with wild-type mice. In addition, a special diet was used for the task of studying the origins of a 680 nm autofluorescent signal that was previously observed in colon. The study found close agreement among each of the modalities and with histology. All four modalities were capable of identifying diseased tissue accurately. The OCT and LSCM images provided complementary structural information about the tissue, while the autofluorescence signal measured by LIF and LSCM provided biochemical information. OCT and LIF were performed in vivo and nondestructively, while the LSCM and histology required extraction of the tissue. The magnitude of the 680 nm signal correlates with chlorophyll content in the mouse diet, suggesting that the autofluorescent compound is a dietary metabolite.
ISSN:1559-128X
DOI:10.1364/AO.45.003049