Genetic analysis of pbp2x in clinical Streptococcus pneumoniae isolates in Quebec, Canada

Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations. Methods:...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2005-06, Vol.55 (6), p.832-839
Main Authors: Granger, Dominic, Boily-Larouche, Geneviève, Turgeon, Pierre, Weiss, Karl, Roger, Michel
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Antibiotics. Antiinfectious agents. Antiparasitic agents
beta-Lactam Resistance
Biological and medical sciences
cefotaxime resistance
Humans
Medical sciences
Molecular Sequence Data
penicillin resistance
penicillin-binding proteins
Penicillin-Binding Proteins - chemistry
Penicillin-Binding Proteins - genetics
Pharmacology. Drug treatments
pneumococci
serotype
Streptococcus pneumoniae - drug effects
Streptococcus pneumoniae - genetics
Structure-Activity Relationship
β-lactams
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Summary:Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2x of penicillin-resistant Streptococcus pneumoniae isolates across the province of Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations. Methods: The pbp2x genomic region encompassing codons 178–703, which includes the entire region of the transpeptidase domain, was sequenced and compared for 52 clinical isolates comprising 20 penicillin-susceptible (PSSP), 20 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci. Results: The degree of diversity within PBP2x correlated with increased resistance to β-lactam antibiotics. There were an average of 5.0 ± 1.8 mutations in PSSP, 37.9 ± 4.4 in PISP, and 63.0 ± 2.0 in PRSP isolates when compared with the control penicillin-susceptible strain R6. At least six distinct amino acid profiles were identified among PISP strains isolated in Quebec. In contrast, all PRSP isolates shared a similar pattern of altered amino acids compared with the sequence from susceptible strains. Conclusions: These data will be useful in future studies to monitor the genetic changes associated with the emergence and spread of β-lactam resistance in Quebec.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/dki118